现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
JOURNAL OF MODERN LABORATORY MEDICINE
2015年
3期
43-45,49
,共4页
唐海霞%王成%路美玲%张春妮
唐海霞%王成%路美玲%張春妮
당해하%왕성%로미령%장춘니
肾透明细胞癌%血清%miR-193a-3p%定量 PCR%早期诊断%分子标志物
腎透明細胞癌%血清%miR-193a-3p%定量 PCR%早期診斷%分子標誌物
신투명세포암%혈청%miR-193a-3p%정량 PCR%조기진단%분자표지물
renal clear cell carcinoma%serum%miR-193a-3p%quantitativereverse-transcription PCR%early diagnosis%molecular biomarker
目的:检测肾透明细胞癌患者血清中 miR-193a-3p 的表达水平,探讨其作为肾透明细胞癌分子诊断指标的可能性。方法收集南京军区南京总医院2010~2014年期间107例住院肾透明细胞癌患者(TNM 分期Ⅰ期76例、Ⅱ期16例、Ⅲ期2例、Ⅳ期8例和未知5例)未经治疗前血清样本,实时荧光定量 PCR(qRT-PCR)方法检测 miR-193a-3p 含量,并以107例年龄、性别匹配的非癌健康人作对照。ROC 曲线分析血清 miR-193a-3p 水平对肾透明细胞癌的早期诊断价值。结果qRT-PCR 检测证实,miR-193a-3p 在总体患者血清中的含量显著高于对照组(3.294±1.526 vs 1.944±0.600,P=0.000)。TNM Ⅰ期(3.411±1.676 vs 1.944±0.600,P =0.000)、Ⅱ期(2.926±0.927 vs 1.944±0.600,P =0.001)和Ⅳ期患者(2.926±0.894 vs 1.944±0.600,P =0.000)血清中 miR-193a-3p 的表达水平均明显高于对照组。用于诊断肾透明细胞癌早期(Ⅰ期)的血清 miR-193a-3p ROC 曲线下面积(AUCROC)为0.820(95% CI,0.756~0.883),敏感度为80.3%,特异度为93.5%。结论miR-193a-3p 在肾透明细胞癌患者血清中的水平显著升高,并有较高的早期诊断准确性,有望作为该病早期诊断的血清学指标。
目的:檢測腎透明細胞癌患者血清中 miR-193a-3p 的錶達水平,探討其作為腎透明細胞癌分子診斷指標的可能性。方法收集南京軍區南京總醫院2010~2014年期間107例住院腎透明細胞癌患者(TNM 分期Ⅰ期76例、Ⅱ期16例、Ⅲ期2例、Ⅳ期8例和未知5例)未經治療前血清樣本,實時熒光定量 PCR(qRT-PCR)方法檢測 miR-193a-3p 含量,併以107例年齡、性彆匹配的非癌健康人作對照。ROC 麯線分析血清 miR-193a-3p 水平對腎透明細胞癌的早期診斷價值。結果qRT-PCR 檢測證實,miR-193a-3p 在總體患者血清中的含量顯著高于對照組(3.294±1.526 vs 1.944±0.600,P=0.000)。TNM Ⅰ期(3.411±1.676 vs 1.944±0.600,P =0.000)、Ⅱ期(2.926±0.927 vs 1.944±0.600,P =0.001)和Ⅳ期患者(2.926±0.894 vs 1.944±0.600,P =0.000)血清中 miR-193a-3p 的錶達水平均明顯高于對照組。用于診斷腎透明細胞癌早期(Ⅰ期)的血清 miR-193a-3p ROC 麯線下麵積(AUCROC)為0.820(95% CI,0.756~0.883),敏感度為80.3%,特異度為93.5%。結論miR-193a-3p 在腎透明細胞癌患者血清中的水平顯著升高,併有較高的早期診斷準確性,有望作為該病早期診斷的血清學指標。
목적:검측신투명세포암환자혈청중 miR-193a-3p 적표체수평,탐토기작위신투명세포암분자진단지표적가능성。방법수집남경군구남경총의원2010~2014년기간107례주원신투명세포암환자(TNM 분기Ⅰ기76례、Ⅱ기16례、Ⅲ기2례、Ⅳ기8례화미지5례)미경치료전혈청양본,실시형광정량 PCR(qRT-PCR)방법검측 miR-193a-3p 함량,병이107례년령、성별필배적비암건강인작대조。ROC 곡선분석혈청 miR-193a-3p 수평대신투명세포암적조기진단개치。결과qRT-PCR 검측증실,miR-193a-3p 재총체환자혈청중적함량현저고우대조조(3.294±1.526 vs 1.944±0.600,P=0.000)。TNM Ⅰ기(3.411±1.676 vs 1.944±0.600,P =0.000)、Ⅱ기(2.926±0.927 vs 1.944±0.600,P =0.001)화Ⅳ기환자(2.926±0.894 vs 1.944±0.600,P =0.000)혈청중 miR-193a-3p 적표체수평균명현고우대조조。용우진단신투명세포암조기(Ⅰ기)적혈청 miR-193a-3p ROC 곡선하면적(AUCROC)위0.820(95% CI,0.756~0.883),민감도위80.3%,특이도위93.5%。결론miR-193a-3p 재신투명세포암환자혈청중적수평현저승고,병유교고적조기진단준학성,유망작위해병조기진단적혈청학지표。
Objective To determine the levels of miR-193a-3p in serum from patients with renal clear cell carcinoma,and eval-uate the potential of miR-193a-3p asa molecular biomarker of renal clear cell carcinoma.Methods Serum samples were taken from 107 untreated hospitalized patients with renal clear cell carcinoma (TNM Ⅰ grade 76 cases,Ⅱ grade 16 cases,Ⅲ grade 2 cases,Ⅳ grade 8 cases and unknown 5 cases)in Nanjing General Hospital from 2010 to 2014 year and 107 age-and gender-matched healthy individuals to determine the level of miR-193a-3p by quantitative reverse-transcription PCR (qRT-PCR). The early diagnostic value of miR-193a-3p for renal clear cell carcinoma was assessed by ROC curve.Results qRT-PCR confirmed that serum miR-193a-3p expression levels was significantly elevated in cancer than in normal controls (3.294± 1.526 vs 1.944±0.600,P =0.000).TNM Ⅰ grade (3.411±1.676 vs 1.944±0.600,P =0.000),Ⅱ grade (2.926±0.927 vs 1.944±0.600,P =0.001),Ⅳ grade(2.926±0.894 vs 1.944±0.600,P =0.000)is significantly higher than that in con-trol group.The area under the ROC curve (AUC)of miR-193a-3p was 0.820 (95% CI,0.756 ~ 0.883),demonstratinga high sensitivity (80.3%)and specificity (93.5%)for the early diagnosis of renal clear cell carcinoma.Conclusion MiR-193a-3p content was significantly elevated in serum from renal clear cell carcinoma and has a high accuracy in diagnosing ear-ly cancer,which holds potential as molecular biomarker for renal clear cell carcinoma.