CAJ | 학술논문

目的：利用 xCELLigence 实时细胞检测系统快速有效检测人和大鼠气道平滑肌细胞的倍增时间。方法将用胶原酶-胰酶消化法分离获得并用免疫荧光鉴定的大鼠气道平滑肌细胞以及购买获得的人类气道平滑肌细胞，按照3000个／孔的密度种植于 xCELLigence 检测系统布满芯片的 E-plate 培养板内，记录增殖情况100 h 后，用 RTCA Software Package 2.0软件计算细胞倍增时间。结果利用 xCELLigence 实时细胞检测系统获得人气道平滑肌细胞的倍增时间为23.96±0.47 h，与文献资料提供的数据一致，大鼠气道平滑肌细胞的倍增时间为18.62±0.15 h，计算过程简单省时。结论细胞实时分析系统可以有效便捷检测贴壁细胞的倍增时间，为以气道平滑肌细胞为主要研究对象的呼吸疾病基础研究提供实验参考数据和方法。
목적：이용 xCELLigence 실시세포검측계통쾌속유효검측인화대서기도평활기세포적배증시간。방법장용효원매-이매소화법분리획득병용면역형광감정적대서기도평활기세포이급구매획득적인류기도평활기세포，안조3000개／공적밀도충식우 xCELLigence 검측계통포만심편적 E-plate 배양판내，기록증식정황100 h 후，용 RTCA Software Package 2.0연건계산세포배증시간。결과이용 xCELLigence 실시세포검측계통획득인기도평활기세포적배증시간위23.96±0.47 h，여문헌자료제공적수거일치，대서기도평활기세포적배증시간위18.62±0.15 h，계산과정간단성시。결론세포실시분석계통가이유효편첩검측첩벽세포적배증시간，위이기도평활기세포위주요연구대상적호흡질병기출연구제공실험삼고수거화방법。
Objective To detect the doubling time of airway smooth muscle cells of human and rat by the xCELLigence in-strument,a real time cellular analyzer.Methods The airway smooth muscle cells were separated by collagenase-pancreatin digestion from the rat airway.Then,added to the different holes of E-plate of xCELLigence instrument with the planting destiny of 3 000 cells/well.and so were the human airway smooth muscle cells.The E-plate was then placed on the xCELLi-gence instrument to monitor cell proliferation for 100 hours to calculate the doubling time by using the RTCA Software Package 2.0 software.Results The doubling time of human airway smooth muscle cell calculated by the real time cellular analyzer was 23.96±0.47 h,which was consistent with the data provided by the reference.The doubling time of rat airway smooth muscle cell was 18.62±0.15 h,and the computational process was simple,time-saving and also effective.Conclusion The xCELLigence instrument can be used to calculate doubling time of airway smooth muscle cells of human and rat, which provides experimental methods and reference data for the basic respiratory disease research.