世界最新医学信息文摘（连续型电子期刊）
世界最新醫學信息文摘（連續型電子期刊）
세계최신의학신식문적（련속형전자기간）
World Latest Medicine Information
2015年
17期
3-3,2
,共2页
丁峰山%何时义%王爱妍%付鑫%杨东君%凌敏
丁峰山%何時義%王愛妍%付鑫%楊東君%凌敏
정봉산%하시의%왕애연%부흠%양동군%릉민
鸟分枝杆菌%PhoP基因%克隆%分析
鳥分枝桿菌%PhoP基因%剋隆%分析
조분지간균%PhoP기인%극륭%분석
Mycobacterium birds%PhoP gene%Clone%Analysis
目的：对鸟分枝杆菌调控因子PhoP的基因序列和氨基酸序列进行生物信息学分析。方法：利用PCR技术扩增鸟分枝杆菌HIV/AIDS临床分离株PhoP基因，与pEASY-T1载体连接后测序，运用生物信息学工具对其序列进行分析。结果：PCR产物显示一条<为720bp的条带，测序结果与GenBank数据库中鸟分枝杆菌104株PhoP基因序列完全一致。该分离株PhoP的基因和氨基酸序列与结核分枝杆菌H37Rv比对，同源性分别为86%和94%。分离株PhoP基因编码的氨基酸数目为239个氨基酸，其中从136到239位氨基酸为DNA结合区。结论：成功克隆出鸟分枝杆菌HIV/AIDS临床分离株的PhoP基因。该分离株PhoP的氨基酸序列与结核分枝杆菌具有高度的同源性。
目的：對鳥分枝桿菌調控因子PhoP的基因序列和氨基痠序列進行生物信息學分析。方法：利用PCR技術擴增鳥分枝桿菌HIV/AIDS臨床分離株PhoP基因，與pEASY-T1載體連接後測序，運用生物信息學工具對其序列進行分析。結果：PCR產物顯示一條<為720bp的條帶，測序結果與GenBank數據庫中鳥分枝桿菌104株PhoP基因序列完全一緻。該分離株PhoP的基因和氨基痠序列與結覈分枝桿菌H37Rv比對，同源性分彆為86%和94%。分離株PhoP基因編碼的氨基痠數目為239箇氨基痠，其中從136到239位氨基痠為DNA結閤區。結論：成功剋隆齣鳥分枝桿菌HIV/AIDS臨床分離株的PhoP基因。該分離株PhoP的氨基痠序列與結覈分枝桿菌具有高度的同源性。
목적：대조분지간균조공인자PhoP적기인서렬화안기산서렬진행생물신식학분석。방법：이용PCR기술확증조분지간균HIV/AIDS림상분리주PhoP기인，여pEASY-T1재체련접후측서，운용생물신식학공구대기서렬진행분석。결과：PCR산물현시일조<위720bp적조대，측서결과여GenBank수거고중조분지간균104주PhoP기인서렬완전일치。해분리주PhoP적기인화안기산서렬여결핵분지간균H37Rv비대，동원성분별위86%화94%。분리주PhoP기인편마적안기산수목위239개안기산，기중종136도239위안기산위DNA결합구。결론：성공극륭출조분지간균HIV/AIDS림상분리주적PhoP기인。해분리주PhoP적안기산서렬여결핵분지간균구유고도적동원성。
Objective: To conduct the bioinformatics analysis of the Mycobacterium bird regulation PhoP gene sequences and the amino acid sequencing. Methods:PCR technique was used to amplify Mycobacterium HIV/AIDS PhoP gene. After connect-ed with pEASY-T1 vector, the sequence was tested. Bioinformatics tool was utilized to analyze the sequences. Results: PCR products showed an article of 720bp. Sequence results in the GenBank database bird showed that 104 strains of Mycobacterium PhoP gene sequences were exactly the same with those of the PhoP genes. The PhoP gene and amino acid sequences of isolates of Mycobacterium tuberculosis H37Rv were aligned, with the homology of 86% and 94% respectively. The number of amino acids isolated from PhoP gene coding is 239. And amino acids from 136 to 239 were DNA-binding domain. Conclusion:A clin-ical isolated PhoP gene of a Mycobacterium bird HIV/AIDS was successfully cloned. The PhoP amino acid sequences of it have a high degree of homology with the Mycobacterium tuberculosis.
