CAJ | 학술논문

建立了超声萃取-高效液相色谱-质谱(LC-MS/ MS)同时测定鱼肉中7种磺胺类抗生素(磺胺、磺胺对甲氧嘧啶、磺胺间甲氧嘧啶、磺胺甲基嘧啶、磺胺甲噁唑、磺胺二甲嘧啶、磺胺吡啶)和1种磺胺增效剂(甲氧苄啶)的方法。采用乙酸钠缓冲液：乙腈(1：1, V/ V)混合液进行超声萃取,以 SAX/ PSA 双层柱与 HLB 柱组成复合柱进行净化和富集,以 HPLC-MS/ MS 进行分析。提取方法有效降低鱼肉中的基质效应,回收率为80%~117%,检出限<1μg/ kg。方法应用于市售鱼肉中磺胺的检测。
건립료초성췌취-고효액상색보-질보(LC-MS/ MS)동시측정어육중7충광알류항생소(광알、광알대갑양밀정、광알간갑양밀정、광알갑기밀정、광알갑오서、광알이갑밀정、광알필정)화1충광알증효제(갑양변정)적방법。채용을산납완충액：을정(1：1, V/ V)혼합액진행초성췌취,이 SAX/ PSA 쌍층주여 HLB 주조성복합주진행정화화부집,이 HPLC-MS/ MS 진행분석。제취방법유효강저어육중적기질효응,회수솔위80%~117%,검출한<1μg/ kg。방법응용우시수어육중광알적검측。
A method of simultaneously determining 7 sulfonamides, such as sulfanilamide, sulfameter, sulfamonomethoxine, sulfamerazine, sulfamethoxzole, sulfamethazine and sulfapyridine, and one sulfonamide potentiator (trimethoprim) in fish muscle by ultrasonic extraction-liquid chromatography-mass spectrometry (HPLC-MS / MS) was developed. Mixed solution of pH 4. 0 0. 1 M sodium acetate buffer and acetonitrile (1:1, V/ V) was used as extraction carrier during ultrasonic extraction. Extracts were purified and enriched on SAX/ PSA -HLB tandem cartridges. Final sample was analyzed by HPLC-MS / MS. Matrix effects were effectively removed and the recoveries for antibiotics were at the range of 80% ~ 117% , with limits of detection lower than 1 μg / kg. The method was used to determining sulfonamides in commercial fish muscles.