CAJ | 학술논문

该文研究从小麦面粉提取的小麦酯酶粗酶液的纯化、冻干和其有机磷农药的最低检测限。确定了用饱和度为30%和60%的硫酸铵对小麦酯酶粗酶液进行分段盐析，纯化后酶液的比酶活是纯化前的2.4倍；最佳保护剂为5%的聚乙二醇，它不仅可以有效保护纯化后的小麦酯酶在冷冻干燥过程中不失活，而且还具有良好的稳定性；最后确定了冻干小麦酯酶酶粉中敌敌畏、敌百虫、毒死蜱、乐果和辛硫磷等5种有机磷农药最低检测限分别为0.01 mg/kg、0.02 mg/kg、0.05 mg/kg、0.07 mg/kg和0.03 mg/kg，均低于国家规定农药最大残留量，冻干酶粉对敌敌畏灵敏度最佳。
해문연구종소맥면분제취적소맥지매조매액적순화、동간화기유궤린농약적최저검측한。학정료용포화도위30%화60%적류산안대소맥지매조매액진행분단염석，순화후매액적비매활시순화전적2.4배；최가보호제위5%적취을이순，타불부가이유효보호순화후적소맥지매재냉동간조과정중불실활，이차환구유량호적은정성；최후학정료동간소맥지매매분중활활외、활백충、독사비、악과화신류린등5충유궤린농약최저검측한분별위0.01 mg/kg、0.02 mg/kg、0.05 mg/kg、0.07 mg/kg화0.03 mg/kg，균저우국가규정농약최대잔류량，동간매분대활활외령민도최가。
Purification,freeze–driedof wheat esterasecrude enzyme solution extracted from wheat flour and the minimum detection limitoforganophosphorus pesticides were studied. The saturation of 30% and 60% ammonium sulfate was used to treat thecrude enzyme solution by fractional salting out method,specific activityof purified enzyme solution was 2.4 times as much as thecrude enzyme solution;optimal protection agent was 5% polyethylene glycol,which notonlycould effectively keep the purified wheat esterase active in the freeze–drying process,but also had good stability;The results showed that lowest detection limitsof the freeze–dried wheat flour esterase enzyme to dichlorvos, trichlorfonchlorpyrifos,dimethoate,phoxim andother fiveorganophosphorus pesticides were 0.01 mg/kg,0.02 mg/kg,0.05 mg/kg,0.07 mg/kg and 0.03 mg/kg,which were less than the state pesticide maximum residue levels and the dichlorvos was the best sensitivity to Lyophilized enzyme powder.