广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2015年
11期
65-67
,共3页
曹雪杰%高明波%朴晶晶%廖颖玲
曹雪傑%高明波%樸晶晶%廖穎玲
조설걸%고명파%박정정%료영령
白藜芦醇%萃取%双水相%乙醇/ 磷酸氢二钾
白藜蘆醇%萃取%雙水相%乙醇/ 燐痠氫二鉀
백려호순%췌취%쌍수상%을순/ 린산경이갑
resveratrol%extraction%aqueous two-phase%ethanol/ dipotassium phosphate
葡萄酿酒过程中会产生大量的副产品,主要包括:葡萄籽,葡萄皮和葡萄茎;这些副产品约占葡萄总成分的20%。这些副产品是葡萄酿酒后的下脚料,一般被当做废弃物。白藜芦醇却主要存在于以上副产品中。其具有抗菌、抗癌、抗氧化、抑制血小板凝集和舒张血管、提高机体免疫力等特性。本实验利用双水相萃取的方法萃取“双优红”葡萄籽中的白藜芦醇,萃取过程绿色简洁,比传统有机溶剂萃取法有更好的萃取效果。从16种双水相体系中优选出最佳双水相体系乙醇/磷酸氢二钾。在单因素实验基础上,通过四因素水平对比实验优化白藜芦醇的萃取条件为:料液比为1:15,温度为60℃,乙醇萃取浓度为70%,萃取时间为2.5 h,在此最佳工艺条件下白藜芦醇的萃取得量为0.484%。
葡萄釀酒過程中會產生大量的副產品,主要包括:葡萄籽,葡萄皮和葡萄莖;這些副產品約佔葡萄總成分的20%。這些副產品是葡萄釀酒後的下腳料,一般被噹做廢棄物。白藜蘆醇卻主要存在于以上副產品中。其具有抗菌、抗癌、抗氧化、抑製血小闆凝集和舒張血管、提高機體免疫力等特性。本實驗利用雙水相萃取的方法萃取“雙優紅”葡萄籽中的白藜蘆醇,萃取過程綠色簡潔,比傳統有機溶劑萃取法有更好的萃取效果。從16種雙水相體繫中優選齣最佳雙水相體繫乙醇/燐痠氫二鉀。在單因素實驗基礎上,通過四因素水平對比實驗優化白藜蘆醇的萃取條件為:料液比為1:15,溫度為60℃,乙醇萃取濃度為70%,萃取時間為2.5 h,在此最佳工藝條件下白藜蘆醇的萃取得量為0.484%。
포도양주과정중회산생대량적부산품,주요포괄:포도자,포도피화포도경;저사부산품약점포도총성분적20%。저사부산품시포도양주후적하각료,일반피당주폐기물。백려호순각주요존재우이상부산품중。기구유항균、항암、항양화、억제혈소판응집화서장혈관、제고궤체면역력등특성。본실험이용쌍수상췌취적방법췌취“쌍우홍”포도자중적백려호순,췌취과정록색간길,비전통유궤용제췌취법유경호적췌취효과。종16충쌍수상체계중우선출최가쌍수상체계을순/린산경이갑。재단인소실험기출상,통과사인소수평대비실험우화백려호순적췌취조건위:료액비위1:15,온도위60℃,을순췌취농도위70%,췌취시간위2.5 h,재차최가공예조건하백려호순적췌취득량위0.484%。
The wine making process produces a large quantity of grape secondary products, which include grape seeds, skins, and stems and so on. These byproducts comprise approximately 20% of the harvested grapes. All the byproducts are only scraps from the grape wine, generally treated as waste. Resveratrol, exists mainly in the above byproducts, owning a number of functions, such as antibacterial, anti-cancer, anti-oxidation function. It can also inhibit platelet aggregation, bring vasodilation and improve the body immunity. The method of aqueous two-phase extraction was used to extract the resveratrol in the grape Shuangyouhong seeds. The whole extraction is green and simple, which was far better than the traditional organic solvent extraction. Of the 16 kinds of aqueous two-phase systems, the best one of ethanol/ dipotassium phosphate system was optimized. On the basis of single factor experiment and the following four factors level contrast experiment, the extraction conditions of resveratrol were optimized as follows: 70% ethanol, solid to liquid ratio of 1:15, extraction for 2. 5 h at 60 ℃. The content of resveratrol in the Shuangyouhong grape seeds was 0. 484%.