CAJ | 학술논문

探讨7,12-二甲基苯并蒽(DMBA)对肝星状细胞活化的影响。 DMBA(1μmol·L-1)处理人肝星状细胞株 LX-224 h后, Western blot、 ELISA 和实时定量 PCR 观察肝星状细胞活化标志蛋白α-SMA、 TGF-β1、 CYP1A1和 CYP1B1蛋白表达和分泌量以及 mRNA 表达。研究发现,与对照组相比, DMBA 处理后的 LX-2中α-SMA 蛋白和 mRNA 表达均显著升高(P<0.05), TGF-β1分泌量(P<0.05)和 mRNA 表达(P<0.01)也显著升高。此外, DMBA 处理的 LX-2中 CYP1A1和 CYP1B1的 mRNA 表达也显著升高(P<0.05)。结果表明 DMBA 可活化人肝星状细胞,可能与诱导 LX-2中 CYP1A1和 CYP1B1表达有关。
탐토7,12-이갑기분병은(DMBA)대간성상세포활화적영향。 DMBA(1μmol·L-1)처리인간성상세포주 LX-224 h후, Western blot、 ELISA 화실시정량 PCR 관찰간성상세포활화표지단백α-SMA、 TGF-β1、 CYP1A1화 CYP1B1단백표체화분비량이급 mRNA 표체。연구발현,여대조조상비, DMBA 처리후적 LX-2중α-SMA 단백화 mRNA 표체균현저승고(P<0.05), TGF-β1분비량(P<0.05)화 mRNA 표체(P<0.01)야현저승고。차외, DMBA 처리적 LX-2중 CYP1A1화 CYP1B1적 mRNA 표체야현저승고(P<0.05)。결과표명 DMBA 가활화인간성상세포,가능여유도 LX-2중 CYP1A1화 CYP1B1표체유관。
The effects of 7,12-dimethyibenz[a] thrancene (DMBA) on human hepatic stellate cell activation were investigated. The human hepatic stellate cell lines LX-2 was cultured with DMBA (1 μmol·L-1 ) for 24 h. The protein expression of α smooth muscle actin (α-SMA), a marker of hepatic stellate cell activation was detected by western blot. Transformation Growth Factor beta1 (TGF-β1 ) level in culture medium was observed by enzyme linked immunosorbent assay (ELISA) method. The mRNA expressions of α-SMA, TGF-β1 , CYP1A1 and CYP1B1 in LX-2 were assayed by real-time polymerase chain reaction (RT-PCR). Compared with control group, the mRNA expression and protein of α-SMA and TGF-β1 were significantly increased by DMAB (P<0. 05 and 0. 01). The mRNA expression of CYP1A1 and CYP1B1 were also increased by DMBA ( P <0. 05). The results suggested that DMBA could activate human hepatic stellate cells. The induction of CYP1A1 and CYP1B1 may be the possible mechanism of hepatic stellate cells activation by DMBA.