目的 探讨去盆腔神经对大鼠结肠动力与结肠黏膜瞬时受体电位通道A1(TRPA1)的影响.方法 将96只成年大鼠按随机数字表法分为3组:(1)对照组18只,大鼠不做特殊处理,仅常规饲养.(2)假手术组39只,大鼠开腹后剥离出盆腔神经,缝合切口,关闭腹腔.(3)手术组39只,大鼠开腹后分离出盆腔神经,显微剪剪断,缝合切口,关闭腹腔.通过计算各组大鼠术后第1、3、7天结肠组织和粪便中铬(51Cr)分布的几何中心(GC)检测结肠传输功能.运用Western blot检测各组大鼠术后第1、3、7天近端及远端结肠黏膜TRPA1的表达.符合正态分布的计量资料以(x)±s表示,多组间比较采用重复测量的方差分析,两两比较采用LSD检验.结果 假手术组大鼠51Cr的GC值术后第1、3、7天分别为5.8±0.9、7.5±0.5、7.3±0.5,3者比较,差异有统计学意义(F =9.508,P＜0.05).手术组大鼠51Cr的GC值术后第1、3、7天分别为4.9±0.4、5.6±0.4、6.4±0.8,3者比较,差异有统计学意义(F=11.689,P＜0.05).假手术组和手术组大鼠51Cr的GC值术后第1、3天分别比较,差异均有统计学意义(=2.227,7.144,P＜0.05);术后7天两组比较,差异无统计学意义(t=2.162,P＞0.05).Western blot检测结果显示:对照组大鼠近端结肠黏膜中TRPA1相对表达量术后第1、3、7天分别为1.00 ±0.05、1.00±0.07、1.00±0.06,3者比较,差异无统计学意义(F=0.055,P＞0.05).假手术组大鼠近端结肠黏膜中TRPA1相对表达量术后第1、3、7天分别为0.78 ±0.09、0.94 ±0.08、0.95 ±0.12,3者比较,差异有统计学意义(F=5.651,P＜0.05).手术组大鼠近端结肠黏膜中TRPA1相对表达量术后第1、3、7天分别为0.37 ±0.12、0.89 ±0.10、0.92 ±0.14,3者比较,差异有统计学意义(F=41.005,P＜0.05).对照组、假手术组和手术组大鼠近端结肠黏膜中TRPA1相对表达量术后第1天比较,差异有统计学意义(F=73.497,P＜0.05);对照组分别与假手术组和手术组比较,差异均有统计学意义(t=4.224,11.954,P＜0.05);手术组和假手术组比较,差异有统计学意义(t=7.730,P＜0.05).对照组、假手术组和手术组大鼠近端结肠黏膜中TRPA1相对表达量术后第3、7天比较,差异均无统计学意义(F=2.087,0.656,P＞0.05).对照组大鼠远端结肠黏膜中TRPA1相对表达量术后第1、3、7天分别为1.00±0.05、1.00±0.07、1.00±0.06,3者比较,差异无统计学意义(F=0.055,P＞0.05).假手术组大鼠远端结肠黏膜中TRPA1相对表达量术后第1、3、7天分别为0.68 ±0.11、0.98±0.12、1.11 ±0.16,3者比较,差异有统计学意义(F=16.975,P＜0.05).手术组大鼠远端结肠黏膜中TRPA1相对表达量术后第1、3、7天分别为0.39±0.12、0.78 ±0.10、1.06 ±0.13,3者比较,差异有统计学意义(F=50.417,P＜0.05).对照组、假手术组和手术组大鼠远端结肠黏膜中TRPA1相对表达量术后第1、3天比较,差异均有统计学意义(F =58.773,8.680,P ＜0.05).术后第1天,对照组分别与假手术组和手术组比较,差异均有统计学意义(t=5.706,10.837,P＜0.05);手术组和假手术组比较,差异有统计学意义(t=5.131,P＜0.05).术后第3天,对照组与假手术组比较,差异无统计学意义(t=0.166,P＞0.05);对照组与手术组比较,差异有统计学意义(t=3.694,P＜0.05);假手术组和手术组比较,差异有统计学意义(t=3.528,P＜0.05).对照组、假手术组和手术组大鼠远端结肠黏膜中TRPA1相对表达量术后第7天比较,差异无统计学意义(F=1.319,P＞0.05).结论 去盆腔神经支配后,大鼠结肠动力减弱,远端结肠黏膜中TRPA1表达下调,但随时间推移可逐渐恢复,两者在恢复趋势上存在一致性.
目的 探討去盆腔神經對大鼠結腸動力與結腸黏膜瞬時受體電位通道A1(TRPA1)的影響.方法 將96隻成年大鼠按隨機數字錶法分為3組:(1)對照組18隻,大鼠不做特殊處理,僅常規飼養.(2)假手術組39隻,大鼠開腹後剝離齣盆腔神經,縫閤切口,關閉腹腔.(3)手術組39隻,大鼠開腹後分離齣盆腔神經,顯微剪剪斷,縫閤切口,關閉腹腔.通過計算各組大鼠術後第1、3、7天結腸組織和糞便中鉻(51Cr)分佈的幾何中心(GC)檢測結腸傳輸功能.運用Western blot檢測各組大鼠術後第1、3、7天近耑及遠耑結腸黏膜TRPA1的錶達.符閤正態分佈的計量資料以(x)±s錶示,多組間比較採用重複測量的方差分析,兩兩比較採用LSD檢驗.結果 假手術組大鼠51Cr的GC值術後第1、3、7天分彆為5.8±0.9、7.5±0.5、7.3±0.5,3者比較,差異有統計學意義(F =9.508,P＜0.05).手術組大鼠51Cr的GC值術後第1、3、7天分彆為4.9±0.4、5.6±0.4、6.4±0.8,3者比較,差異有統計學意義(F=11.689,P＜0.05).假手術組和手術組大鼠51Cr的GC值術後第1、3天分彆比較,差異均有統計學意義(=2.227,7.144,P＜0.05);術後7天兩組比較,差異無統計學意義(t=2.162,P＞0.05).Western blot檢測結果顯示:對照組大鼠近耑結腸黏膜中TRPA1相對錶達量術後第1、3、7天分彆為1.00 ±0.05、1.00±0.07、1.00±0.06,3者比較,差異無統計學意義(F=0.055,P＞0.05).假手術組大鼠近耑結腸黏膜中TRPA1相對錶達量術後第1、3、7天分彆為0.78 ±0.09、0.94 ±0.08、0.95 ±0.12,3者比較,差異有統計學意義(F=5.651,P＜0.05).手術組大鼠近耑結腸黏膜中TRPA1相對錶達量術後第1、3、7天分彆為0.37 ±0.12、0.89 ±0.10、0.92 ±0.14,3者比較,差異有統計學意義(F=41.005,P＜0.05).對照組、假手術組和手術組大鼠近耑結腸黏膜中TRPA1相對錶達量術後第1天比較,差異有統計學意義(F=73.497,P＜0.05);對照組分彆與假手術組和手術組比較,差異均有統計學意義(t=4.224,11.954,P＜0.05);手術組和假手術組比較,差異有統計學意義(t=7.730,P＜0.05).對照組、假手術組和手術組大鼠近耑結腸黏膜中TRPA1相對錶達量術後第3、7天比較,差異均無統計學意義(F=2.087,0.656,P＞0.05).對照組大鼠遠耑結腸黏膜中TRPA1相對錶達量術後第1、3、7天分彆為1.00±0.05、1.00±0.07、1.00±0.06,3者比較,差異無統計學意義(F=0.055,P＞0.05).假手術組大鼠遠耑結腸黏膜中TRPA1相對錶達量術後第1、3、7天分彆為0.68 ±0.11、0.98±0.12、1.11 ±0.16,3者比較,差異有統計學意義(F=16.975,P＜0.05).手術組大鼠遠耑結腸黏膜中TRPA1相對錶達量術後第1、3、7天分彆為0.39±0.12、0.78 ±0.10、1.06 ±0.13,3者比較,差異有統計學意義(F=50.417,P＜0.05).對照組、假手術組和手術組大鼠遠耑結腸黏膜中TRPA1相對錶達量術後第1、3天比較,差異均有統計學意義(F =58.773,8.680,P ＜0.05).術後第1天,對照組分彆與假手術組和手術組比較,差異均有統計學意義(t=5.706,10.837,P＜0.05);手術組和假手術組比較,差異有統計學意義(t=5.131,P＜0.05).術後第3天,對照組與假手術組比較,差異無統計學意義(t=0.166,P＞0.05);對照組與手術組比較,差異有統計學意義(t=3.694,P＜0.05);假手術組和手術組比較,差異有統計學意義(t=3.528,P＜0.05).對照組、假手術組和手術組大鼠遠耑結腸黏膜中TRPA1相對錶達量術後第7天比較,差異無統計學意義(F=1.319,P＞0.05).結論 去盆腔神經支配後,大鼠結腸動力減弱,遠耑結腸黏膜中TRPA1錶達下調,但隨時間推移可逐漸恢複,兩者在恢複趨勢上存在一緻性.
목적 탐토거분강신경대대서결장동력여결장점막순시수체전위통도A1(TRPA1)적영향.방법 장96지성년대서안수궤수자표법분위3조:(1)대조조18지,대서불주특수처리,부상규사양.(2)가수술조39지,대서개복후박리출분강신경,봉합절구,관폐복강.(3)수술조39지,대서개복후분리출분강신경,현미전전단,봉합절구,관폐복강.통과계산각조대서술후제1、3、7천결장조직화분편중락(51Cr)분포적궤하중심(GC)검측결장전수공능.운용Western blot검측각조대서술후제1、3、7천근단급원단결장점막TRPA1적표체.부합정태분포적계량자료이(x)±s표시,다조간비교채용중복측량적방차분석,량량비교채용LSD검험.결과 가수술조대서51Cr적GC치술후제1、3、7천분별위5.8±0.9、7.5±0.5、7.3±0.5,3자비교,차이유통계학의의(F =9.508,P＜0.05).수술조대서51Cr적GC치술후제1、3、7천분별위4.9±0.4、5.6±0.4、6.4±0.8,3자비교,차이유통계학의의(F=11.689,P＜0.05).가수술조화수술조대서51Cr적GC치술후제1、3천분별비교,차이균유통계학의의(=2.227,7.144,P＜0.05);술후7천량조비교,차이무통계학의의(t=2.162,P＞0.05).Western blot검측결과현시:대조조대서근단결장점막중TRPA1상대표체량술후제1、3、7천분별위1.00 ±0.05、1.00±0.07、1.00±0.06,3자비교,차이무통계학의의(F=0.055,P＞0.05).가수술조대서근단결장점막중TRPA1상대표체량술후제1、3、7천분별위0.78 ±0.09、0.94 ±0.08、0.95 ±0.12,3자비교,차이유통계학의의(F=5.651,P＜0.05).수술조대서근단결장점막중TRPA1상대표체량술후제1、3、7천분별위0.37 ±0.12、0.89 ±0.10、0.92 ±0.14,3자비교,차이유통계학의의(F=41.005,P＜0.05).대조조、가수술조화수술조대서근단결장점막중TRPA1상대표체량술후제1천비교,차이유통계학의의(F=73.497,P＜0.05);대조조분별여가수술조화수술조비교,차이균유통계학의의(t=4.224,11.954,P＜0.05);수술조화가수술조비교,차이유통계학의의(t=7.730,P＜0.05).대조조、가수술조화수술조대서근단결장점막중TRPA1상대표체량술후제3、7천비교,차이균무통계학의의(F=2.087,0.656,P＞0.05).대조조대서원단결장점막중TRPA1상대표체량술후제1、3、7천분별위1.00±0.05、1.00±0.07、1.00±0.06,3자비교,차이무통계학의의(F=0.055,P＞0.05).가수술조대서원단결장점막중TRPA1상대표체량술후제1、3、7천분별위0.68 ±0.11、0.98±0.12、1.11 ±0.16,3자비교,차이유통계학의의(F=16.975,P＜0.05).수술조대서원단결장점막중TRPA1상대표체량술후제1、3、7천분별위0.39±0.12、0.78 ±0.10、1.06 ±0.13,3자비교,차이유통계학의의(F=50.417,P＜0.05).대조조、가수술조화수술조대서원단결장점막중TRPA1상대표체량술후제1、3천비교,차이균유통계학의의(F =58.773,8.680,P ＜0.05).술후제1천,대조조분별여가수술조화수술조비교,차이균유통계학의의(t=5.706,10.837,P＜0.05);수술조화가수술조비교,차이유통계학의의(t=5.131,P＜0.05).술후제3천,대조조여가수술조비교,차이무통계학의의(t=0.166,P＞0.05);대조조여수술조비교,차이유통계학의의(t=3.694,P＜0.05);가수술조화수술조비교,차이유통계학의의(t=3.528,P＜0.05).대조조、가수술조화수술조대서원단결장점막중TRPA1상대표체량술후제7천비교,차이무통계학의의(F=1.319,P＞0.05).결론 거분강신경지배후,대서결장동력감약,원단결장점막중TRPA1표체하조,단수시간추이가축점회복,량자재회복추세상존재일치성.
Objective To investigate the effects of pelvic nerve transection on the colonic motility and the expression of transit receptor potential ankyrin 1 (TRPA1) in the colon mucosa.Methods Ninety-six Sprague-Dawley rats were divided into 3 groups based on a random number table:(1) 18 rats in the control group remained untreated and were fed regularly;(2) 39 rats in the sham operation group received laparotomy,and the pelvic nerves were stripped;(3) 39 rats in the operation group received laparotomy with pelvic nerve transection before abdominal closure.Colonic transit was assessed respectively at postoperative day 1,3,and 7 by injecting and calculating the geometric center (GC) of the distribution of 51Cr after 3 hours of propagation.The expression of TRPA1 in the colonic mucosa was determined by Western blot at postoperative day 1,3,7.Data with normal distribution were expressed by (x)± s,and were analyzed using the repeated measures ANOVA or LSD test.Results The GC values of the distribution of 51Cr in the sham operation group at postoperative day 1,3,7 were 5.8 ± 0.9,7.5 ± 0.5,7.3 ± 0.5,with a significant difference (F =9.508,P ＜ 0.05).The GC values of the distribution of 51Cr in the operation group at postoperative day 1,3,7 were 4.9 ± 0.4,5.6 ± 0.4,6.4 ± 0.8,with a significant difference (F =11.689,P ＜ 0.05).There were significant differences in the GC values of the distribution of 51 Cr at postoperative day 1 and 3 between the sham operation group and the operation group (t =2.227,7.144,P ＜ 0.05),while no significant difference was detected at postoperative day 7 (t =2.162,P ＞ 0.05).The results of Western blot showed that the relative expressions of TRPA1 in the proximal part of the colon at postoperative day 1,3,7 were 1.00 ± 0.05,1.00 ± 0.07,1.00 ± 0.06 in the control group,with a significant difference (F =0.055,P ＞ 0.05).The relative expressions of TRPA1 in the proximal part of the colon at postoperative day 1,3,7 were 0.78 ± 0.09,0.94 ± 0.08,0.95 ± 0.12 in the sham operation group,with a significant difference (F =5.651,P ＜ 0.05).The relative expressions of TRPA1 in the proximal part of the colon at postoperative day 1,3,7 were 0.37 ± 0.12,0.89 ± 0.10,0.92 ± 0.14 in the operation group,with a significant difference (F =41.005,P ＜0.05).There was significant difference in the relative expressions of TRPA1 in the proximal part of the colon among the 3 groups at postoperative day 1 (F =73.497,P ＜ 0.05),while significant differences were respectively detected between the control group and the sham operation group and the operation group at postoperative day 1 (t =4.224,11.954,P ＜ 0.05),and significant difference between the operation group and the sham operation group was also observed (t =7.730,P ＜ 0.05).There was no significant difference in the relative expression of TRPA1 in the proximal part of the colon among the 3 groups between day 3 and day 7 (F =2.087,0.656,P ＞ 0.05).The relative expressions of TRPA1 in the distal part of the colon at postoperative day 1,3,7 were 1.00 ± 0.05,1.00 ± 0.07,1.00 ± 0.06 in the control group,with no significant difference (F =0.055,P ＞ 0.05).The relative expressions of TRPA1 in the distal part of the colon at postoperative day 1,3,7were 0.68 ±0.11,0.98 ±0.12,1.11 ±0.16 in the sham operation group,with a significant difference (F =16.975,P ＜ 0.05).The relative expressions of TRPA1 in the distal part of the colon at postoperative day 1,3,7 were 0.39 ± 0.12,0.78 ± 0.10,1.06 ± 0.13 in the operation group,with a significant difference (F =50.417,P ＜ 0.05).There were significant differences in the relative expression of TRPA1 in the distal part of the colon among the 3 groups between day 1 and day 3 (F =58.773,8.680,P ＜ 0.05),while significant differences were respectively detected between the control group and the sham operation group and the operation group at postoperative day 1 (t =5.706,10.837,P ＜ 0.05),and significant difference was also detected between the operation group and the sham operation group (t =5.131,P ＜ 0.05).There was no significant difference in the relative expression of TRPA1 in the distal part of the colon between the control group and the sham operation group at postoperative day 3 (t =0.166,P ＞ 0.05),while significant differences were respectively detected between the control group and the operation group and between the sham operation group and the operation group at postoperative day 3 (t =3.694,3.528,P ＜ 0.05).There was no significant difference in the relative expression of the TRPA1 in the distal part of the colon between the 3 groups at postoperative day 7 (F =1.319,P ＞ 0.05).Conclusions Injury to pelvic nerves adversely affects colonic transit and expression of TRPA1 in mucosa.With a compensatory mechanism from the intestinal itself,these alterations in intestinal motility function normalize over time suggesting expression of TRPA1 in mucosa plays a crucial role in the recovery of intestinal motility function.
