CAJ | 학술논문

目的：探讨人参皂苷 Rg3对人低分化鼻咽癌细胞系 CNE -2体外血管生成拟态及迁移能力的影响。方法用不同浓度（0、38、76和114μmol/ L）的 Rg3处理 CNE -2细胞后，体外管道形成抑制实验检测 Rg3对CNE -2细胞管道形成能力的影响；Transwell 小室法检测 Rg3对 CNE -2细胞迁移能力的影响；Western blot 法检测 Rg3对 CNE -2细胞中 COX -2、HIF -1α、VEGF 和 Fascin1蛋白表达的影响。结果人参皂苷 Rg3能抑制 CNE -2细胞体外管道形成的能力，其管状结构数量与 Rg3浓度呈负相关（P ＜0.01）；Rg3浓度大于76μmol/ L 时可以抑制 CNE -2细胞的迁移能力，与0μmol/ L Rg3比较差异有统计学意义（P ＜0.05）；且 Rg3对 COX -2、HIF -1α、VEGF 和 Fascin1蛋白表达的影响趋势分别与 Rg3对血管生成拟态和迁移能力的变化趋势呈正相关（ P ＜0.05）。结论人参皂苷 Rg3能抑制 CNE -2细胞体外血管生成拟态及迁移能力，其分子机制可能与抑制 CNE -2细胞中COX -2、HIF -1α、VEGF 和 Fascin1蛋白的表达有关。
목적：탐토인삼조감 Rg3대인저분화비인암세포계 CNE -2체외혈관생성의태급천이능력적영향。방법용불동농도（0、38、76화114μmol/ L）적 Rg3처리 CNE -2세포후，체외관도형성억제실험검측 Rg3대CNE -2세포관도형성능력적영향；Transwell 소실법검측 Rg3대 CNE -2세포천이능력적영향；Western blot 법검측 Rg3대 CNE -2세포중 COX -2、HIF -1α、VEGF 화 Fascin1단백표체적영향。결과인삼조감 Rg3능억제 CNE -2세포체외관도형성적능력，기관상결구수량여 Rg3농도정부상관（P ＜0.01）；Rg3농도대우76μmol/ L 시가이억제 CNE -2세포적천이능력，여0μmol/ L Rg3비교차이유통계학의의（P ＜0.05）；차 Rg3대 COX -2、HIF -1α、VEGF 화 Fascin1단백표체적영향추세분별여 Rg3대혈관생성의태화천이능력적변화추세정정상관（ P ＜0.05）。결론인삼조감 Rg3능억제 CNE -2세포체외혈관생성의태급천이능력，기분자궤제가능여억제 CNE -2세포중COX -2、HIF -1α、VEGF 화 Fascin1단백적표체유관。
Objective To study the inhibitory effects of 20(R)- ginsenoside Rg3 on vasculogenic mimicry and migration of human nasopharyngeal carcinoma CNE - 2 cell line in vitro. Methods CNE - 2 cells treated with different concentrations of Rg3(0 μmol·L - 1 ,38 μmol·L - 1 ,76 μmol·L - 1 and 114 μmol·L - 1 )were assayed with transwell assay and anti - angiogenic test for testing the potential of migration and tube - like structure(TLSs)formation,respec-tively. Meanwhile,the expression of COX - 2,HIF - 1α,VEGF and Fascin1 proteins were detected by Western blotting. Results The formation of TLSs in CNE - 2 cells were significantly inhibited by Rg3 in concentration dependent manners (P < 0. 01);so was the migration ability of CNE - 2 cells(P < 0. 05). At the same time,the expression of COX - 2, HIF - 1α,VEGF and Fascin1 in CNE - 2 cells were down - regulated by Rg3. A significantly positive correlation between the expression of Fascin1 and the migration ability was observed(P < 0. 05),so was the significantly positive correlation between the expression of COX - 2,HIF - 1α and VEGF and the inhibition of the TLS formation(P < 0. 05). Conclusion Rg3 can inhibit the vasculogenic mimicry and migration of CNE - 2 cells in vitro,via the down - regulation of COX - 2, HIF - 1α,VEGF and Fascin1.