中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2015年
17期
60-63
,共4页
王娟%李燕%赵骏达%马俊旗
王娟%李燕%趙駿達%馬俊旂
왕연%리연%조준체%마준기
人乳头状瘤病毒L1壳蛋白%人端粒酶RNA基因%宫颈癌
人乳頭狀瘤病毒L1殼蛋白%人耑粒酶RNA基因%宮頸癌
인유두상류병독L1각단백%인단립매RNA기인%궁경암
Human papilloma virus L1 protein%Human telomerase RNA gene%Cervical cancer
目的：探讨人乳头状瘤病毒L1壳蛋白和人端粒酶RNA基因联合检测在宫颈癌筛查中的意义。方法选取2012年10月~2014年10月新疆医科大学第一附属医院行宫颈癌筛查的患者627例，先行液基细胞学检查，后行阴道镜检查并取活检，同时行人乳头状瘤病毒L1壳蛋白检测和人端粒酶RNA基因检测。分析不同病症间临床指标的变异情况。结果未见上皮病变或恶性改变(NILM)、意义不明的不典型鳞状细胞与不典型腺细胞(ASC-US)、不除外高度鳞状上皮内病变的的不典型鳞状细胞(ASC-H)、低度鳞状上皮内病变(LSIL)、高度鳞状上皮内病变(HSIL)、鳞状细胞癌(SCC)、腺癌(AC)，人乳头状瘤病毒L1壳蛋白阳性表达率分别为18.6%、42.9%、51.0%、80.6%、47.4%、5.9%、0.0%，差异有高度统计学意义(χ2=93.770，P<0.01)，呈先升高后降低趋势，在LSIL达到最高点，在AC降至最低点；人端粒酶RNA基因阳性表达率分别为0.0%、19.9%、36.3%、51.6%、94.7%、100.0%、100.0%，差异有高度统计学意义(χ2=279.564，P<0.01)，呈持续升高趋势，在SCC与AC达到最高点。依正常、炎症、宫颈上皮内瘤变(CIN)Ⅰ期、CINⅡ期、CINⅢ期、宫颈癌顺序，人乳头状瘤病毒L1壳蛋白阳性表达率分别为18.6%、46.1%、80.6%、53.3%、25.0%、3.6%，差异有高度统计学意义(χ2=93.015，P<0.01)，呈先升高后降低趋势，在CINⅠ期达到最高点，在宫颈癌降至最低点；人端粒酶RNA基因阳性表达率分别为0.0%、26.4%、51.6%、93.3%、100.0%、100.0%，差异有高度统计学意义(χ2=269.582，P<0.01)，呈持续升高趋势，在CINⅢ期达到最高点。结论人乳头状瘤病毒L1壳蛋白联合人端粒酶RNA基因检测，在宫颈癌前病变中具有较高的指导价值。
目的：探討人乳頭狀瘤病毒L1殼蛋白和人耑粒酶RNA基因聯閤檢測在宮頸癌篩查中的意義。方法選取2012年10月~2014年10月新疆醫科大學第一附屬醫院行宮頸癌篩查的患者627例，先行液基細胞學檢查，後行陰道鏡檢查併取活檢，同時行人乳頭狀瘤病毒L1殼蛋白檢測和人耑粒酶RNA基因檢測。分析不同病癥間臨床指標的變異情況。結果未見上皮病變或噁性改變(NILM)、意義不明的不典型鱗狀細胞與不典型腺細胞(ASC-US)、不除外高度鱗狀上皮內病變的的不典型鱗狀細胞(ASC-H)、低度鱗狀上皮內病變(LSIL)、高度鱗狀上皮內病變(HSIL)、鱗狀細胞癌(SCC)、腺癌(AC)，人乳頭狀瘤病毒L1殼蛋白暘性錶達率分彆為18.6%、42.9%、51.0%、80.6%、47.4%、5.9%、0.0%，差異有高度統計學意義(χ2=93.770，P<0.01)，呈先升高後降低趨勢，在LSIL達到最高點，在AC降至最低點；人耑粒酶RNA基因暘性錶達率分彆為0.0%、19.9%、36.3%、51.6%、94.7%、100.0%、100.0%，差異有高度統計學意義(χ2=279.564，P<0.01)，呈持續升高趨勢，在SCC與AC達到最高點。依正常、炎癥、宮頸上皮內瘤變(CIN)Ⅰ期、CINⅡ期、CINⅢ期、宮頸癌順序，人乳頭狀瘤病毒L1殼蛋白暘性錶達率分彆為18.6%、46.1%、80.6%、53.3%、25.0%、3.6%，差異有高度統計學意義(χ2=93.015，P<0.01)，呈先升高後降低趨勢，在CINⅠ期達到最高點，在宮頸癌降至最低點；人耑粒酶RNA基因暘性錶達率分彆為0.0%、26.4%、51.6%、93.3%、100.0%、100.0%，差異有高度統計學意義(χ2=269.582，P<0.01)，呈持續升高趨勢，在CINⅢ期達到最高點。結論人乳頭狀瘤病毒L1殼蛋白聯閤人耑粒酶RNA基因檢測，在宮頸癌前病變中具有較高的指導價值。
목적：탐토인유두상류병독L1각단백화인단립매RNA기인연합검측재궁경암사사중적의의。방법선취2012년10월~2014년10월신강의과대학제일부속의원행궁경암사사적환자627례，선행액기세포학검사，후행음도경검사병취활검，동시행인유두상류병독L1각단백검측화인단립매RNA기인검측。분석불동병증간림상지표적변이정황。결과미견상피병변혹악성개변(NILM)、의의불명적불전형린상세포여불전형선세포(ASC-US)、불제외고도린상상피내병변적적불전형린상세포(ASC-H)、저도린상상피내병변(LSIL)、고도린상상피내병변(HSIL)、린상세포암(SCC)、선암(AC)，인유두상류병독L1각단백양성표체솔분별위18.6%、42.9%、51.0%、80.6%、47.4%、5.9%、0.0%，차이유고도통계학의의(χ2=93.770，P<0.01)，정선승고후강저추세，재LSIL체도최고점，재AC강지최저점；인단립매RNA기인양성표체솔분별위0.0%、19.9%、36.3%、51.6%、94.7%、100.0%、100.0%，차이유고도통계학의의(χ2=279.564，P<0.01)，정지속승고추세，재SCC여AC체도최고점。의정상、염증、궁경상피내류변(CIN)Ⅰ기、CINⅡ기、CINⅢ기、궁경암순서，인유두상류병독L1각단백양성표체솔분별위18.6%、46.1%、80.6%、53.3%、25.0%、3.6%，차이유고도통계학의의(χ2=93.015，P<0.01)，정선승고후강저추세，재CINⅠ기체도최고점，재궁경암강지최저점；인단립매RNA기인양성표체솔분별위0.0%、26.4%、51.6%、93.3%、100.0%、100.0%，차이유고도통계학의의(χ2=269.582，P<0.01)，정지속승고추세，재CINⅢ기체도최고점。결론인유두상류병독L1각단백연합인단립매RNA기인검측，재궁경암전병변중구유교고적지도개치。
Objective To investigate significance of human papilloma virus L1 protein and human telomerase RNA gene combined detection in cervical cancer screening. Methods From October 2012 to October 2014, in the First Af-filiated Hospital of Xinjiang Medical University, 627 patients given cervical cancer screening were selected. Firstly, the patients received liquid based cytology, then they received colposcopy and biopsy, at the same time, they also received human papilloma virus L1 protein detection and human telomerase RNA gene detection. The variation of clinical indi-cators were analyzed between different conditions. Results According to sequence of NILM, ASC-US, ASC-H, LSIL, HSIL, SCC, AC, positive expression ratio of human papilloma virus L1 protein were 18.6%, 42.9%, 51.0%, 80.6%, 47.4%, 5.9%, 0.0%, the difference was statistically significant (χ2=93.770, P<0.01), which showed increasing at first and then decreasing, it reached highest point at LSIL , and reached lowest point at AC; positive expression ratio of hu-man telomerase RNA gene were 0.0%, 19.9%, 36.3%, 51.6%, 94.7%, 100.0%, 100.0%, the difference was statistically significant (χ2= 279.564, P< 0.01), which showed continue increasing, it reached highest point at SCC and AC. Ac-cording to sequence of normal, inflammation, CIN phase Ⅰ, CIN phase II, CIN phase Ⅲ, cervical cancer, positive ex-pression ratio of human papilloma virus L1 protein were 18.6%, 46.1%, 80.6%, 53.3%, 25.0%, 3.6%, the difference was statistically significant (χ2 = 93.015, P< 0.01), which showed increasing at first and then decreasing, it reached highest point at CIN phase Ⅰ, and it reached lowest point at cervical cancer; positive expression ratio of human telomerase RNA gene were 0.0%, 26.4%, 51.6%, 93.3%, 100.0%, 100.0%, the difference was statistically significant (χ2 = 269.582, P < 0.01), which showed continue increasing, it reached highest point at CIN phase II. Conclusion Human papilloma virus L1 protein and human telomerase RNA gene combined detection has higher guiding value in cervical precancerous lesions.
