中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2015年
17期
16-19
,共4页
胃癌%环氧合酶-2%血管内皮细胞生长因子%血管内皮生长因子C%免疫组织化学
胃癌%環氧閤酶-2%血管內皮細胞生長因子%血管內皮生長因子C%免疫組織化學
위암%배양합매-2%혈관내피세포생장인자%혈관내피생장인자C%면역조직화학
Gastric cancer%COX-2%VEGF%VEGF-C%Immunohistochemistry
目的:探讨环氧合酶-2(COX-2)、血管内皮细胞生长因子(VEGF)、血管内皮生长因子C(VEGF-C)蛋白在胃癌组织中的表达及意义。方法采用免疫组化SP法检测COX-2、VEGF、VEGF-C蛋白在70例胃癌标本和30例正常胃黏膜中的表达情况。结果COX-2、VEGF、VEGF-C蛋白在胃癌细胞胞质中呈棕黄色表达,表达率分别为71.4%、62.9%、55.7%,在正常胃黏膜中的表达率为13.3%、6.7%、16.7%。不同年龄、性别、肿瘤部位、肿瘤大小的COX-2、VEGF、VEGF-C蛋白阳性表达率差异无统计学意义(P>0.05);不同肿瘤TNM分期、淋巴转移的COX-2、VEGF、VEGF-C蛋白阳性表达率差异有统计学意义(P<0.05);不同病理分级的COX-2蛋白阳性表达率差异无统计学意义(P>0.05),而VEGF与VEGF-C蛋白的阳性表达率差异有统计学意义(P<0.05)。 COX-2与VEGF、VEGF-C蛋白,VEGF与VEGF-C蛋白在胃癌组织中的表达呈正相关(P<0.05)。结论 COX-2、VEGF、VEGF-C蛋白在胃癌组织中高表达,COX-2的表达与VEGF、VEGF-C的表达相关,COX-2可能通过调节VEGF、VEGF-C蛋白的表达从而调节胃癌的发生、发展过程。
目的:探討環氧閤酶-2(COX-2)、血管內皮細胞生長因子(VEGF)、血管內皮生長因子C(VEGF-C)蛋白在胃癌組織中的錶達及意義。方法採用免疫組化SP法檢測COX-2、VEGF、VEGF-C蛋白在70例胃癌標本和30例正常胃黏膜中的錶達情況。結果COX-2、VEGF、VEGF-C蛋白在胃癌細胞胞質中呈棕黃色錶達,錶達率分彆為71.4%、62.9%、55.7%,在正常胃黏膜中的錶達率為13.3%、6.7%、16.7%。不同年齡、性彆、腫瘤部位、腫瘤大小的COX-2、VEGF、VEGF-C蛋白暘性錶達率差異無統計學意義(P>0.05);不同腫瘤TNM分期、淋巴轉移的COX-2、VEGF、VEGF-C蛋白暘性錶達率差異有統計學意義(P<0.05);不同病理分級的COX-2蛋白暘性錶達率差異無統計學意義(P>0.05),而VEGF與VEGF-C蛋白的暘性錶達率差異有統計學意義(P<0.05)。 COX-2與VEGF、VEGF-C蛋白,VEGF與VEGF-C蛋白在胃癌組織中的錶達呈正相關(P<0.05)。結論 COX-2、VEGF、VEGF-C蛋白在胃癌組織中高錶達,COX-2的錶達與VEGF、VEGF-C的錶達相關,COX-2可能通過調節VEGF、VEGF-C蛋白的錶達從而調節胃癌的髮生、髮展過程。
목적:탐토배양합매-2(COX-2)、혈관내피세포생장인자(VEGF)、혈관내피생장인자C(VEGF-C)단백재위암조직중적표체급의의。방법채용면역조화SP법검측COX-2、VEGF、VEGF-C단백재70례위암표본화30례정상위점막중적표체정황。결과COX-2、VEGF、VEGF-C단백재위암세포포질중정종황색표체,표체솔분별위71.4%、62.9%、55.7%,재정상위점막중적표체솔위13.3%、6.7%、16.7%。불동년령、성별、종류부위、종류대소적COX-2、VEGF、VEGF-C단백양성표체솔차이무통계학의의(P>0.05);불동종류TNM분기、림파전이적COX-2、VEGF、VEGF-C단백양성표체솔차이유통계학의의(P<0.05);불동병리분급적COX-2단백양성표체솔차이무통계학의의(P>0.05),이VEGF여VEGF-C단백적양성표체솔차이유통계학의의(P<0.05)。 COX-2여VEGF、VEGF-C단백,VEGF여VEGF-C단백재위암조직중적표체정정상관(P<0.05)。결론 COX-2、VEGF、VEGF-C단백재위암조직중고표체,COX-2적표체여VEGF、VEGF-C적표체상관,COX-2가능통과조절VEGF、VEGF-C단백적표체종이조절위암적발생、발전과정。
Objective To study the protein expression and significance of cyclooxygenase-2 (COX-2), vascular endothe-lial growth factor (VEGF) and vascular endothelial growth factor C (VEGF-C) in gastric cancer. Methods The expres-sion levels of COX-2, VEGF and VEGF-C were detected by using immunohistochemical SP method in 70 cases of gas-tric cancer and 30 cases of normal gastric mucosa. Results The positive expression rates of COX-2, VEGF and VEGF-C in gastric cancer were 71.4%, 62.9% and 55.7%; and were 13.3%, 6.7% and 16.7% in normal gastric mucosa. There were no statistical significant differences in positive expression rates of COX-2, VEGF and VEGF-C among patients with different age, sex, tumor site and size (P>0.05). There were statistical significant differences among patients with dif-ferent TNM stages and lymph nodes (P<0.05). There were no statistical significant difference in positive expression rate of COX-2 among patients with different pathological grading (P> 0.05); while there were statistical significant differ-ences in VEGF and VEGF-C (P< 0.05). There were positive correlation between the expression of COX-2 and VEGF, VEGF-C;VEGF and VEGF-C in gastric cancer (P<0.05). Conclusion There are over-expressions of COX-2, VEGF and VEGF-C in gastric cancer tissues. The expression of COX-2 and VEGF, VEGF-C is correlated. COX-2 may regu-late the gastric cancer occurrence and progress through regulating the protein expression of VEGF and VEGF-C.