热带病与寄生虫学
熱帶病與寄生蟲學
열대병여기생충학
TROPICAL DISEASES AND PARASITOLOGY
2015年
2期
63-65
,共3页
贝类多糖%HepG2.2.15细胞%JAK-STAT信号通路
貝類多糖%HepG2.2.15細胞%JAK-STAT信號通路
패류다당%HepG2.2.15세포%JAK-STAT신호통로
Shellfish polysaccharide%HepG2.2.15 cells%JAK-STAT transduction pathways
目的：了解贝类多糖对HepG2.2.15细胞JAK-STAT信号转导途径分子表达的影响。方法用RT-PCR的方法检测经贝类多糖处理的HepG2.2.15细胞在不同时间段的STAT1和STAT2 mRNA表达的水平。结果 RT-PCR结果显示经贝类多糖处理后HepG2.2.15细胞STAT1和STAT2 mRNA表达水平明显升高，与对照组比较差异有统计学意义（P<0.05），表明HepG2.2.15细胞对贝类多糖有应答。结论贝类多糖对HBV有一定的抑制作用，其作用机制可能与INF-α类似。
目的：瞭解貝類多糖對HepG2.2.15細胞JAK-STAT信號轉導途徑分子錶達的影響。方法用RT-PCR的方法檢測經貝類多糖處理的HepG2.2.15細胞在不同時間段的STAT1和STAT2 mRNA錶達的水平。結果 RT-PCR結果顯示經貝類多糖處理後HepG2.2.15細胞STAT1和STAT2 mRNA錶達水平明顯升高，與對照組比較差異有統計學意義（P<0.05），錶明HepG2.2.15細胞對貝類多糖有應答。結論貝類多糖對HBV有一定的抑製作用，其作用機製可能與INF-α類似。
목적：료해패류다당대HepG2.2.15세포JAK-STAT신호전도도경분자표체적영향。방법용RT-PCR적방법검측경패류다당처리적HepG2.2.15세포재불동시간단적STAT1화STAT2 mRNA표체적수평。결과 RT-PCR결과현시경패류다당처리후HepG2.2.15세포STAT1화STAT2 mRNA표체수평명현승고，여대조조비교차이유통계학의의（P<0.05），표명HepG2.2.15세포대패류다당유응답。결론패류다당대HBV유일정적억제작용，기작용궤제가능여INF-α유사。
Objective To understand the influence of shellfish polysaccharide on the molecular expres?sion of JAK-STAT transduction pathways in HepG2.2.15 cells. Methods After treatment with shellfish poly?saccharides, the expression levels of STAT1 and STAT2 mRNA in HepG2.2.15 cells in different phase were detected by reverse transcription polymerase chain reaction (RT-PCR). Results RT-PCR results showed that the expression levels of the STAT1and STAT2 mRNAs in HepG2.2.15 cells treated with shellfish polysac?charides were significantly up-regulated as compared with the control group (P<0.05), which suggested the HepG2.2.15 could respond to the shellfish polysaccharides. Conclusion The shellfish polysaccharide has cer?tain inhibitory effect on hepatitis B virus(HBV), and its mechanism looks similar to the INF-α.
