环球中医药
環毬中醫藥
배구중의약
GLOBAL TCM
2015年
6期
697-701
,共5页
蒋静%李元文%张丰川%蔡玲玲%聂晶%李天娇%张力元%陈雪燕%李根茂%葛东宇
蔣靜%李元文%張豐川%蔡玲玲%聶晶%李天嬌%張力元%陳雪燕%李根茂%葛東宇
장정%리원문%장봉천%채령령%섭정%리천교%장력원%진설연%리근무%갈동우
甘石青黛膏%B淋巴细胞瘤-2蛋白%Bax蛋白%细胞凋亡%亚急性湿疹
甘石青黛膏%B淋巴細胞瘤-2蛋白%Bax蛋白%細胞凋亡%亞急性濕疹
감석청대고%B림파세포류-2단백%Bax단백%세포조망%아급성습진
Ganshi Qingdai Cream%B-cell lymphoma-2%Bax protein%Cell apoptosis%Subacute eczema
目的：观察甘石青黛膏对大鼠亚急性湿疹模型皮损淋巴细胞和角质形成细胞B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)蛋白与Bax蛋白表达变化的影响,探究中药外治湿疹的分子生物学依据。方法造模前取大鼠正常皮肤组织,制作成石蜡切片备用,造模成功后分为治疗组和模型组,治疗组采用中药甘石青黛膏干预,模型组无干预,检测三组大鼠Bcl-2与Bax蛋白表达情况。结果造模前大鼠Bcl-2与Bax蛋白表达均呈阳性,且Bcl-2/Bax比率接近于1；治疗组Bcl-2蛋白表达呈弱阳性,模型组Bcl-2蛋白表达呈强阳性,两者相比具有显著统计学差异( P<0．001)；治疗组Bax蛋白表达呈阳性,模型组Bax蛋白表达呈弱阳性,两者相比具有显著统计学差异(P<0．001)；治疗组与模型组Bcl-2/Bax比率相比具有显著统计学差异( P<0．001),后者明显高于前者；治疗组与健康组Bcl-2/Bax比率相比无统计学差(P>0．05)。结论甘石青黛膏具有抑制Bcl-2蛋白表达,促进Bax蛋白表达,使细胞凋亡调控系统趋于平衡的作用。
目的：觀察甘石青黛膏對大鼠亞急性濕疹模型皮損淋巴細胞和角質形成細胞B淋巴細胞瘤-2(B-cell lymphoma-2,Bcl-2)蛋白與Bax蛋白錶達變化的影響,探究中藥外治濕疹的分子生物學依據。方法造模前取大鼠正常皮膚組織,製作成石蠟切片備用,造模成功後分為治療組和模型組,治療組採用中藥甘石青黛膏榦預,模型組無榦預,檢測三組大鼠Bcl-2與Bax蛋白錶達情況。結果造模前大鼠Bcl-2與Bax蛋白錶達均呈暘性,且Bcl-2/Bax比率接近于1；治療組Bcl-2蛋白錶達呈弱暘性,模型組Bcl-2蛋白錶達呈彊暘性,兩者相比具有顯著統計學差異( P<0．001)；治療組Bax蛋白錶達呈暘性,模型組Bax蛋白錶達呈弱暘性,兩者相比具有顯著統計學差異(P<0．001)；治療組與模型組Bcl-2/Bax比率相比具有顯著統計學差異( P<0．001),後者明顯高于前者；治療組與健康組Bcl-2/Bax比率相比無統計學差(P>0．05)。結論甘石青黛膏具有抑製Bcl-2蛋白錶達,促進Bax蛋白錶達,使細胞凋亡調控繫統趨于平衡的作用。
목적：관찰감석청대고대대서아급성습진모형피손림파세포화각질형성세포B림파세포류-2(B-cell lymphoma-2,Bcl-2)단백여Bax단백표체변화적영향,탐구중약외치습진적분자생물학의거。방법조모전취대서정상피부조직,제작성석사절편비용,조모성공후분위치료조화모형조,치료조채용중약감석청대고간예,모형조무간예,검측삼조대서Bcl-2여Bax단백표체정황。결과조모전대서Bcl-2여Bax단백표체균정양성,차Bcl-2/Bax비솔접근우1；치료조Bcl-2단백표체정약양성,모형조Bcl-2단백표체정강양성,량자상비구유현저통계학차이( P<0．001)；치료조Bax단백표체정양성,모형조Bax단백표체정약양성,량자상비구유현저통계학차이(P<0．001)；치료조여모형조Bcl-2/Bax비솔상비구유현저통계학차이( P<0．001),후자명현고우전자；치료조여건강조Bcl-2/Bax비솔상비무통계학차(P>0．05)。결론감석청대고구유억제Bcl-2단백표체,촉진Bax단백표체,사세포조망조공계통추우평형적작용。
Objective To observe the effects on the Bcl-2 and Bax protein expression changes of lymphocytes and keratinocyte cells from subacute eczema lesions in rats intervened by Ganshi Qingdai Cream,and to explore the molecular biology basis of traditional Chinese medicine external treatment of ecze-ma. Methods Removing the normal skin tissue in rats before modeling, and making them into paraffin sections to use. After the success of modeling all the rats are divided into the treatment group and the model group,the treatment group is used by traditional Chinese medicine Ganshi Qingdai Cream to intervene, the model group is without intervention. Detecting the Bcl-2 and Bax protein expression in three groups of rats. Results The Bcl-2 and Bax protein expression of rats are positive before modeling,and the Bcl-2/Bax ratio closes to 1. In treatment group, the Bcl-2 protein expression is weakly positive, and in model group, the Bcl-2 protein expression is strongly positive, there is statistically significant difference between the two groups(P<0. 001). In treatment group,the Bax protein expression is positive,and in model group,the Bax protein expression is weakly positive,the two groups compare with statistically significant difference(P<0. 001). There is statistically significant difference of the Bcl-2/Bax ratio between treating group compared with model group (P<0-001),which is higher than that of the former. There is not statistically difference of Bcl-2/Bax ratio between treating group compared with healthy group ( P>0. 05 ) Conclusions Ganshi Qingdai Cream can inhibit the Bcl-2 protein expression,promot the Bax protein expression,and make the cell apoptosis regulation system tend to balance.
