CAJ | 학술논문

目的：观察参苓白术散加减方对脾虚泄泻模型大鼠结肠黏膜组织水通道蛋白( aqua-porin,AQP)4、AQP8的表达的影响。方法健康雄性Wistar大鼠42只随机分为正常组、模型组、高中低剂量组及西药组6组,采用水环境小平台站立法复合高乳糖饲料喂养的方法复制腹泻模型,模型成功后,灌胃给药7天,取结肠组织,通过免疫组化的方法观察结肠黏膜组织AQP4、AQP8的变化。结果与正常组比较,模型组大鼠结肠黏膜组织AQP4、AQP8的表达明显降低；与模型组比较,高中低剂量组及西药组均可以提高结肠黏膜组织中 AQP4的表达,高低剂量组和西药组可提高AQP8的表达,中剂量组改善不明显。结论参苓白术散加减方可提高脾虚泄泻模型大鼠结肠黏膜组织中的AQP4、AQP8的表达水平,促进水分的吸收,从而达到止泻的目的。
목적：관찰삼령백술산가감방대비허설사모형대서결장점막조직수통도단백( aqua-porin,AQP)4、AQP8적표체적영향。방법건강웅성Wistar대서42지수궤분위정상조、모형조、고중저제량조급서약조6조,채용수배경소평태참입법복합고유당사료위양적방법복제복사모형,모형성공후,관위급약7천,취결장조직,통과면역조화적방법관찰결장점막조직AQP4、AQP8적변화。결과여정상조비교,모형조대서결장점막조직AQP4、AQP8적표체명현강저；여모형조비교,고중저제량조급서약조균가이제고결장점막조직중 AQP4적표체,고저제량조화서약조가제고AQP8적표체,중제량조개선불명현。결론삼령백술산가감방가제고비허설사모형대서결장점막조직중적AQP4、AQP8적표체수평,촉진수분적흡수,종이체도지사적목적。
Objective To investigate the effects ofmodification of prescription of Shenling Baizhu Power on the Expressions of AQP4 and AQP8 proteins in colonic mucosa tissue. Methods Three week-old healthy male rats were randomly dividedinto six groups:control group, model group, high dose group, mid-dle dose group, low dose group and west medicine group. The rats in the model group and treatment groups were given a high lactose diet, and were forced to stand on the multiple platforms which is full of water a-round to establish the type of spleen deficiency diarrhea. The medicine was given once a day for 7days. And then, AQP4 and AQP8 expression changes in the colon mucosa tissue were observed through the meth-od of immunohistochemistry. Results The expressions of AQP4 and AQP8 in the model group significantly-decreasedcompared to that of in the control group. While compared to the model group, the expression of AQP4 in all the treatment groups were significantly increased, and the expression of AQP8 in the high and low dose groups and west medicine group were significantly increased. Conclusion The modification of prescription of Shenling Baizhu Power can antidiarrhea may via the way of increasing the expressions of AQP4 and AQP8 proteins to adjust the water absorption in colonic mucosa tissue of the rats with spleen de-ficiency diarrhea.