CAJ | 학술논문

目的：研究Claudin-4在浆液性卵巢癌组织中的表达及临床病理意义。方法收集2008年10月至2014年5月于宁海县妇幼保健院妇产科行手术切除的43例浆液性卵巢癌及对应癌旁组织，采用实时定量PCR（qRT-PCR）检测Claudin-4 mRNA在浆液性卵巢癌及癌旁组织中的表达，免疫组织化学染色检测癌与癌旁组织中Claudin-4蛋白表达，分析Claudin-4蛋白与肿瘤临床病理资料之间的相关性。结果浆液性卵巢癌组织中Claudin-4 mRNA及蛋白表达水平均较对应癌旁卵巢组织显著升高（ P＜0.05），浆液性卵巢癌组织中高表达Claudin-4蛋白与肿瘤体积增大、淋巴结转移及高TNM分期呈显著正相关（ P＜0.05）。结论 Claudin-4 mRNA及蛋白在浆液性卵巢癌组织中表达上调，高Claudin-4蛋白表达与肿瘤恶性临床病理特征有关；Claudin-4可能成为浆液性卵巢癌早期诊断的重要标志物和生物靶向治疗的有效靶点之一，具有广阔的临床应用前景。
목적：연구Claudin-4재장액성란소암조직중적표체급림상병리의의。방법수집2008년10월지2014년5월우저해현부유보건원부산과행수술절제적43례장액성란소암급대응암방조직，채용실시정량PCR（qRT-PCR）검측Claudin-4 mRNA재장액성란소암급암방조직중적표체，면역조직화학염색검측암여암방조직중Claudin-4단백표체，분석Claudin-4단백여종류림상병리자료지간적상관성。결과장액성란소암조직중Claudin-4 mRNA급단백표체수평균교대응암방란소조직현저승고（ P＜0.05），장액성란소암조직중고표체Claudin-4단백여종류체적증대、림파결전이급고TNM분기정현저정상관（ P＜0.05）。결론 Claudin-4 mRNA급단백재장액성란소암조직중표체상조，고Claudin-4단백표체여종류악성림상병리특정유관；Claudin-4가능성위장액성란소암조기진단적중요표지물화생물파향치료적유효파점지일，구유엄활적림상응용전경。
Objective To investigate the expression and clinical significance of Claudin-4 in human se-rous ovarian cancer.Methods 43 cases of serous ovarian cancer from Oct.2008 to May 2014 were studied.Re-al-time quantitative PCR( qRT-PCR) was applied to detect mRNA expression of Claudin-4 in serous ovarian cancer ( n=43 ) in comparison to the corresponding tumor-adjacent tissues.The protein expression of Claudin-4 was measured by immunohistochemistry( IHC) .Results mRNA and protein expression level of Claudin-4 was signif-icantly higher in serous ovarian cancer tissues than in adjacent normal tissues(P<0.05).The high expression of Claudin-4 protein was also associated with large tumor size, lymphatic metastasis, and advanced TNM stage( P<0.05 ) .Conclusions The expression of Claudin-4 is significantly higher in serous ovarian cancer tissues than in the adjacent normal tissues, and it is associated with clinicopathological features.Claudin-4 may become a new marker in early diagnosis and biological target therapy.