CAJ | 학술논문

目的：研究某院院内临床分离的多重耐药鲍曼不动杆菌中，氨基糖苷类耐药基因及消毒剂基因（qacE△1）的存在情况。方法采用自动化微生物仪VTEK-2对2013年9月26日～2013年12月8日临床分离的10株多重耐药鲍曼不动杆菌进行细菌鉴定和药敏试验部分抗菌药物的敏感性采用纸片扩散法。PCR法检测氨基糖苷类耐药基因及qacE△1基因。结果10株多重耐药鲍曼不动杆菌中，10株（100%）ant（3"）-1基因均阳性，9株（90%）aac（6'）-Ib基因阳性，1株（10%）aac（3）-Ⅳ基因阳性，9株（90%）armA基因阳性。10株（100%）qacE△1基因均阳性。10株多重耐药鲍曼不动杆菌中，7株多重耐药鲍曼不动杆菌来源于ICU重症监护病房，3株来源于神经外重症监护病房。结论院内临床分离的多重耐药鲍曼不动杆菌对氨基糖苷类抗菌药物耐药可能与氨基糖苷类耐药基因有关，临床中应注意消毒剂的选择。
목적：연구모원원내림상분리적다중내약포만불동간균중，안기당감류내약기인급소독제기인（qacE△1）적존재정황。방법채용자동화미생물의VTEK-2대2013년9월26일～2013년12월8일림상분리적10주다중내약포만불동간균진행세균감정화약민시험부분항균약물적민감성채용지편확산법。PCR법검측안기당감류내약기인급qacE△1기인。결과10주다중내약포만불동간균중，10주（100%）ant（3"）-1기인균양성，9주（90%）aac（6'）-Ib기인양성，1주（10%）aac（3）-Ⅳ기인양성，9주（90%）armA기인양성。10주（100%）qacE△1기인균양성。10주다중내약포만불동간균중，7주다중내약포만불동간균래원우ICU중증감호병방，3주래원우신경외중증감호병방。결론원내림상분리적다중내약포만불동간균대안기당감류항균약물내약가능여안기당감류내약기인유관，림상중응주의소독제적선택。
Objective To investigate the prevalence of aminoglycoside resistance genes and disinfectant-resistant genes (qacE△1) in multi-drug resistant Acinetobacter baumannii isolated in a hospital. Methods Bacterial identiifcation and susceptibility testing of 10 strains of multidrug resistant Acinetobacter baumannii which isolated from September 26th, 2013 to December 8th, 2013 were performed by automated microbiology analyzer VITEK-2. Aminoglycoside resistance genes and qacE△1 gene were detected by polymerase chain reaction (PCR). Results Among the 10 strains of multi-drug resistant Acinetobacter baumannii, ant(3")-1 was found with 10 (100%) strains, aac(6')-Ⅰwas identiifed in 9 (90%) strains, aac(3)-Ⅳin 1 (10%) strain, and 9 (90%) strains carried armA gene. There were 7 strains of multi-drug resistant Acinetobacter baumannii were separated from intensive care unit and the rest 3 strains were isolated from neurosurgical intensive care unit. Conclusions The aminoglycoside resistance of multi-drug resistant Acinetobacter baumannii isolated in clinical may be associated with the aminoglycoside resistance genes, and the selection of the disinfectant should be paid much attention in clinical.