CAJ | 학술논문

目的：研究错配修复基因家族成员hMLH1、hMSH2、hMSH6在结直肠癌中的表达及其临床意义。方法采用免疫组化SP法检测72例结直肠癌组织中hMLH1、hMSH2、hMSH6蛋白的表达,并分析三者的相关性及与临床病理特征的关系。结果72例结直肠癌组织hMLH1、hMSH2、hMSH6表达缺失率分别为73．6%、65．3%和43．1%。hMLH1、hMSH2表达缺失率与肿瘤直径、分化程度、Duke's分期、淋巴结转移及远处转移有关（P<0．05, P<0．01),与性别、年龄无关（P>0．05)；hMSH6蛋白表达缺失率与肿瘤分化程度、Duke's分期、淋巴结转移有关（P<0．05),而与性别、年龄、肿瘤直径及远处转移无关（P>0．05)。结直肠癌hMLH1、hMSH2蛋白表达缺失率呈正相关（r=0．295, P<0．05)；hMLH1、hMSH6与hMSH2、hMSH6蛋白表达缺失率无显著相关性（P>0．05)。结论 hMLH1、hMSH2、hMSH6可能在结直肠癌的发生、发展中起重要作用,联合检测对结直肠癌的诊断与治疗有一定价值。
목적：연구착배수복기인가족성원hMLH1、hMSH2、hMSH6재결직장암중적표체급기림상의의。방법채용면역조화SP법검측72례결직장암조직중hMLH1、hMSH2、hMSH6단백적표체,병분석삼자적상관성급여림상병리특정적관계。결과72례결직장암조직hMLH1、hMSH2、hMSH6표체결실솔분별위73．6%、65．3%화43．1%。hMLH1、hMSH2표체결실솔여종류직경、분화정도、Duke's분기、림파결전이급원처전이유관（P<0．05, P<0．01),여성별、년령무관（P>0．05)；hMSH6단백표체결실솔여종류분화정도、Duke's분기、림파결전이유관（P<0．05),이여성별、년령、종류직경급원처전이무관（P>0．05)。결직장암hMLH1、hMSH2단백표체결실솔정정상관（r=0．295, P<0．05)；hMLH1、hMSH6여hMSH2、hMSH6단백표체결실솔무현저상관성（P>0．05)。결론 hMLH1、hMSH2、hMSH6가능재결직장암적발생、발전중기중요작용,연합검측대결직장암적진단여치료유일정개치。
Objective To study the mismatch repair gene family members' hMLH1, hMSH2, hMSH6 expression and the relationship between the three in colon-rectal cancer and its clinical significance. Methods Immunohistochemical SP method was used to detected 72 cases of colon-rectal cancer for tissues hMLH1, hMSH2, hMSH6 protein expression, and a-nalysis of the correlation and pathological features was made. Results For hMLH1, hMSH2, hMSH6 expression in 72 cases of colon-rectal cancer, tissues deletion rate was 73. 6%, 65. 3 and 43. 1%, respectively. In colon-rectal cancer and hMLH1, hMSH2 expression missing rate and protein expression were correlated to tumor size, differentiation, Dukes stage, lymph node metastasis and distant metastasis (P<0. 05), and but not to gender and age (P>0. 05). hMSH6 protein deletion rate was related with degree of tumor differentiation, Dukes stage, lymph node metastasis ( P <0. 05 ) , but not with gender, age, tumor size and distant metastasis (P>0. 05). hMLH1, hMSH2 protein expression was positively correlated with expression missing in colon-rectal cancer in the (r= 0. 295, P<0. 05). hMLH1, hMSH6 and hMSH2, hMSH6 protein expression dele-tion had no significant relationship (P>0. 05). Conclusion hMLH1, hMSH2, hMSH6 may play an important role in the occurrence of colon-rectal cancer, and the combined detection of colon-rectal cancer may have some reference value in diagno-sis and treatment of colon-rectal cancer.