临床儿科杂志
臨床兒科雜誌
림상인과잡지
2015年
6期
535-538
,共4页
病毒性脑炎%肺表面活性物质相关蛋白%血清%脑脊液%儿童
病毒性腦炎%肺錶麵活性物質相關蛋白%血清%腦脊液%兒童
병독성뇌염%폐표면활성물질상관단백%혈청%뇌척액%인동
viral encephalitis%surfactant protein-D%serum%cerebrospinal lfuid%child
目的：探讨病毒性脑炎(VE)患儿血清和脑脊液中肺表面活性物质相关蛋白D(SP-D)的变化。方法采用酶联免疫吸附试验双抗体夹心法分别检测30例VE患儿急性期及恢复期以及12例非脑炎儿童血清和脑脊液中的SP-D，并进行比较分。结果 VE患儿急性期、恢复期以及对照组之间血清和脑脊液SP-D水平差异有统计学意义(F=103.58、118.15，P均<0.01)。其中，VE急性期、恢复期患儿的血清和脑脊液SP-D水平均低于对照组，差异有统计学意义(P<0.01)；VE恢复期血清和脑脊液SP-D水平较VE急性期有所提高，差异有统计学意义(P均<0.01)。VE患儿脑脊液中SP-D水平与脑脊液有核细胞数呈负弱相关(r=－0.317，P=0.014)。结论 SP-D可能参与病毒性脑炎的发病过程，检测血清和脑脊液中的SP-D对VE诊断具有一定的价值。
目的：探討病毒性腦炎(VE)患兒血清和腦脊液中肺錶麵活性物質相關蛋白D(SP-D)的變化。方法採用酶聯免疫吸附試驗雙抗體夾心法分彆檢測30例VE患兒急性期及恢複期以及12例非腦炎兒童血清和腦脊液中的SP-D，併進行比較分。結果 VE患兒急性期、恢複期以及對照組之間血清和腦脊液SP-D水平差異有統計學意義(F=103.58、118.15，P均<0.01)。其中，VE急性期、恢複期患兒的血清和腦脊液SP-D水平均低于對照組，差異有統計學意義(P<0.01)；VE恢複期血清和腦脊液SP-D水平較VE急性期有所提高，差異有統計學意義(P均<0.01)。VE患兒腦脊液中SP-D水平與腦脊液有覈細胞數呈負弱相關(r=－0.317，P=0.014)。結論 SP-D可能參與病毒性腦炎的髮病過程，檢測血清和腦脊液中的SP-D對VE診斷具有一定的價值。
목적：탐토병독성뇌염(VE)환인혈청화뇌척액중폐표면활성물질상관단백D(SP-D)적변화。방법채용매련면역흡부시험쌍항체협심법분별검측30례VE환인급성기급회복기이급12례비뇌염인동혈청화뇌척액중적SP-D，병진행비교분。결과 VE환인급성기、회복기이급대조조지간혈청화뇌척액SP-D수평차이유통계학의의(F=103.58、118.15，P균<0.01)。기중，VE급성기、회복기환인적혈청화뇌척액SP-D수평균저우대조조，차이유통계학의의(P<0.01)；VE회복기혈청화뇌척액SP-D수평교VE급성기유소제고，차이유통계학의의(P균<0.01)。VE환인뇌척액중SP-D수평여뇌척액유핵세포수정부약상관(r=－0.317，P=0.014)。결론 SP-D가능삼여병독성뇌염적발병과정，검측혈청화뇌척액중적SP-D대VE진단구유일정적개치。
Objective To investigate the changes of the pulmonary surfactant protein-D (SP-D) in serum and cerebrospi-nal lfuid in children with viral encephalitis (VE). Methods The levels of SP-D in serum and cerebrospinal lfuid were detected by a double-antibody sandwich enzyme-linked immunosorbent assay and compared in thirty children with VE in acute and con-valescent phases and in 12 children without VE. Results The levels of SP-D in serum and cerebrospinal lfuid between groups of VE acute phase and convalescent phase and no VE were statistically signiifcant (F=103.58,118.15, all P<0.01). The levels of SP-D in serum and cerebrospinal lfuid in children with VE in acute phase and in convalescent phase were signiifcantly lower than children without VE (P<0.01). The levels of SP-D in serum and cerebrospinal lfuid in children with VE in convalescent phase were all signiifcantly higher than those in acute phase (P<0.01). In children with VE, the level of SP-D in cerebrospinal lfuid was weakly correlated negatively with the count of nucleated cells. Conclusions SP-D might be involved in the pathogenesis in VE. The detection of SP-D in serum and cerebrospinal lfuid has a certain value for diagnosis of VE.
