微生物与感染
微生物與感染
미생물여감염
JOURNAL OF MICROBES AND INFECTION
2015年
3期
154-158
,共5页
迟苗苗%宋娟%宋芹芹%于洁%罗小暖%张璐%王欣玲%韩俊
遲苗苗%宋娟%宋芹芹%于潔%囉小暖%張璐%王訢玲%韓俊
지묘묘%송연%송근근%우길%라소난%장로%왕흔령%한준
鼻病毒%非结构蛋白2B%内质网应激%凋亡
鼻病毒%非結構蛋白2B%內質網應激%凋亡
비병독%비결구단백2B%내질망응격%조망
Rhinovirus%Non-structural protein 2B%Endoplasmic reticulum stress%Apoptosis
为探讨鼻病毒非结构蛋白2B诱导内质网应激和细胞凋亡的机制,本研究构建了鼻病毒非结构蛋白2B的真核表达载体p2B‐GFP ,通过转染BHK‐21细胞检测相关标志蛋白的变化情况。结果显示,非结构蛋白2B定位表达于BHK‐21细胞内质网,诱导内质网应激标志蛋白Grp78、CHOP的表达增加,并使活化转录因子6(ATF6)的转录活性增加,还诱导BHK‐21细胞发生核浓缩而凋亡,使凋亡标志蛋白PARP发生降解而减少。结果提示,鼻病毒非结构蛋白2B可诱导细胞发生内质网应激,并经该途径诱导细胞凋亡。
為探討鼻病毒非結構蛋白2B誘導內質網應激和細胞凋亡的機製,本研究構建瞭鼻病毒非結構蛋白2B的真覈錶達載體p2B‐GFP ,通過轉染BHK‐21細胞檢測相關標誌蛋白的變化情況。結果顯示,非結構蛋白2B定位錶達于BHK‐21細胞內質網,誘導內質網應激標誌蛋白Grp78、CHOP的錶達增加,併使活化轉錄因子6(ATF6)的轉錄活性增加,還誘導BHK‐21細胞髮生覈濃縮而凋亡,使凋亡標誌蛋白PARP髮生降解而減少。結果提示,鼻病毒非結構蛋白2B可誘導細胞髮生內質網應激,併經該途徑誘導細胞凋亡。
위탐토비병독비결구단백2B유도내질망응격화세포조망적궤제,본연구구건료비병독비결구단백2B적진핵표체재체p2B‐GFP ,통과전염BHK‐21세포검측상관표지단백적변화정황。결과현시,비결구단백2B정위표체우BHK‐21세포내질망,유도내질망응격표지단백Grp78、CHOP적표체증가,병사활화전록인자6(ATF6)적전록활성증가,환유도BHK‐21세포발생핵농축이조망,사조망표지단백PARP발생강해이감소。결과제시,비병독비결구단백2B가유도세포발생내질망응격,병경해도경유도세포조망。
To investigate whether the non‐structural protein 2B of rhinovirus 16 induces endoplasmic reticulum stress (ERS) and apoptosis ,BHK‐21 cells were transfected with a plasmid p2B‐GFP expressing rhinovirus 16 2B protein .Biomarkers of ERS and apoptosis were detected respectively .The results showed that 2B protein was localized to the ER apparatus .2B protein increased the expressions of ERS marker proteins Grp 78 and CHOP and the transcriptional activity of activating transcription factor 6 (ATF6) .We also found 2B protein induced nuclear condensation associated with apoptosis and degradation of marker protein PARP in BHK‐21 cells .In conclusion ,the rhinovirus 16 2B protein induces both ERS and apoptosis .