干旱地区农业研究
榦旱地區農業研究
간한지구농업연구
AGRICULTURAL RESEARCH IN THE ARID AREAS
2015年
3期
153-158
,共6页
甘草%愈伤组织%双向电泳%蛋白质组学
甘草%愈傷組織%雙嚮電泳%蛋白質組學
감초%유상조직%쌍향전영%단백질조학
Glycyrrhiza uralensis callus%two-dimensional gel electrophoresis%proteomics
以来源于野生乌拉尔甘草的愈伤组织为试材，对甘草愈伤组织蛋白质的提取、SDS－PAGE电泳、上样量、染色方法等关键步骤进行了优化，结果表明：采用改良丙酮法可提高提取液中蛋白质的含量及单向电泳图谱的分辨率；17 cm IPG胶条上样量为450μg时，采用银染法提高了双向电泳图谱的分辨率及分离效果，获得了1132个蛋白点。建立了一套适用于乌拉尔甘草的愈伤组织蛋白质组分析的双向电泳方法。
以來源于野生烏拉爾甘草的愈傷組織為試材，對甘草愈傷組織蛋白質的提取、SDS－PAGE電泳、上樣量、染色方法等關鍵步驟進行瞭優化，結果錶明：採用改良丙酮法可提高提取液中蛋白質的含量及單嚮電泳圖譜的分辨率；17 cm IPG膠條上樣量為450μg時，採用銀染法提高瞭雙嚮電泳圖譜的分辨率及分離效果，穫得瞭1132箇蛋白點。建立瞭一套適用于烏拉爾甘草的愈傷組織蛋白質組分析的雙嚮電泳方法。
이래원우야생오랍이감초적유상조직위시재，대감초유상조직단백질적제취、SDS－PAGE전영、상양량、염색방법등관건보취진행료우화，결과표명：채용개량병동법가제고제취액중단백질적함량급단향전영도보적분변솔；17 cm IPG효조상양량위450μg시，채용은염법제고료쌍향전영도보적분변솔급분리효과，획득료1132개단백점。건립료일투괄용우오랍이감초적유상조직단백질조분석적쌍향전영방법。
Callus of Glycyrrhiza uralensis was used in this research for extraction of total proteins ,SDS -PAGE , loading quantity and staining method ,allowing optimization of the two-dimensional gel electrophoresis (2D-E) technolo-gy .The results showed that improved acetone precipitation could increase the protein contents and image resolution of gel electrophoresis .There were 1 132 protein spots identified using silver staining when the loading quantity of the sample protein was 450 μg/(17 cm IPG trip ) .This research developed a 2D-E method applicable for protein analysis of Gly-cyrrhiz a uralensis callus .
