CAJ | 학술논문

目的：探讨糖尿病肾病（ DN）患者血浆差异蛋白表达情况，为寻找DN敏感血浆蛋白标志物提供依据。方法4例确诊的DN患者（ DN组）和4例健康人群（对照组）作为研究对象，应用荧光差异双向凝胶电泳分析两组血浆蛋白质组差异表达，以上调或下调1．5倍为截断值筛选出差异表达的蛋白质斑点进行质谱分析。结果成功构建DN患者血浆蛋白差异表达双向凝胶电泳图谱，并分析鉴定出6种差异表达蛋白；通过质谱分析和蛋白质数据库（ UniProtKB／Swiss-Prot）比对鉴定出6种蛋白质，分别为补体C3、C4、凝溶胶蛋白前体、纤维蛋白原γ、载脂蛋白E和细胞骨架蛋白16。结论荧光差异双向凝胶电泳可鉴定筛选出DN患者与正常人群差异表达的血浆蛋白质，并为进一步筛选DN血浆蛋白标志物提供依据。
목적：탐토당뇨병신병（ DN）환자혈장차이단백표체정황，위심조DN민감혈장단백표지물제공의거。방법4례학진적DN환자（ DN조）화4례건강인군（대조조）작위연구대상，응용형광차이쌍향응효전영분석량조혈장단백질조차이표체，이상조혹하조1．5배위절단치사선출차이표체적단백질반점진행질보분석。결과성공구건DN환자혈장단백차이표체쌍향응효전영도보，병분석감정출6충차이표체단백；통과질보분석화단백질수거고（ UniProtKB／Swiss-Prot）비대감정출6충단백질，분별위보체C3、C4、응용효단백전체、섬유단백원γ、재지단백E화세포골가단백16。결론형광차이쌍향응효전영가감정사선출DN환자여정상인군차이표체적혈장단백질，병위진일보사선DN혈장단백표지물제공의거。
Objective To investigate the differential expression of plasma protein in patients with diabetic nephropathy ( DN) and to provide a basis for finding diabetic nephropathy sensitive plasma protein markers .Methods Four DN pa-tients and 4 healthy people were included in this study .The plasma of the included patients were extracted and tested for differential expression by using 2-DDIGE.Higher or lower than 1.5 times was considered as the cutoff filter to screen the differentially expressed protein spots which would be analyzed by mass spectrometry .Results We successfully constructed the two-dimensional gel electrophoresis profiles of plasma protein differential expression of patients with DN , and 6 different expression plasma proteins were identified .By mass spectrometry and UniProtKB/Swiss-Prot, we identified 6 proteins, in-cluding complement C3, C4, gelsolin precursor, fibrinogenγ, apolipoprotein E and cytoskeletal protein 16.Conclusion The differential expression plasma protein between DN patients and healthy people can by identified by bidirectional fluores -cence difference gel electrophoresis , which provides the basis for further screening DN plasma protein markers.