CAJ | 학술논문

对桑黄固体和液体发酵培养条件及菌丝多糖的提取工艺进行优化。结果表明：桑黄液体发酵培养基的最佳碳源是玉米粉和黄豆粉,最佳氮源是酵母浸出汁和黄豆粉,最佳碳氮比25∶1,温度28℃,装液量80 mL,转速110 r/min,发酵培养时间9天。以巨菌草为主要基料进行固体发酵培养,桑黄菌丝长势良好。巨菌草发酵培养的桑黄菌丝多糖的最佳提取工艺为温度90℃,料液比1∶40,提取时间1.5 h,以3倍体积的乙醇醇沉多糖的条件下多糖得率最高,达到12.06%,高于其他栽培方式的桑黄菌丝体多糖含量。
대상황고체화액체발효배양조건급균사다당적제취공예진행우화。결과표명：상황액체발효배양기적최가탄원시옥미분화황두분,최가담원시효모침출즙화황두분,최가탄담비25∶1,온도28℃,장액량80 mL,전속110 r/min,발효배양시간9천。이거균초위주요기료진행고체발효배양,상황균사장세량호。거균초발효배양적상황균사다당적최가제취공예위온도90℃,료액비1∶40,제취시간1.5 h,이3배체적적을순순침다당적조건하다당득솔최고,체도12.06%,고우기타재배방식적상황균사체다당함량。
The culture conditions of solid and liquid fermentation of Phellinus linteus and mycelial polysaccharide extraction process are optimized.The result shows that the optimal carbon sources for liquid fermentation medium are corn powder and soybean powder and the optimal nitrogen sources are yeast extract and soybean powder,the best ratio of carbon to nitrogen is 25∶1,temperature is 28 ℃, liquid medium volume is 80 mL,rotating speed is 1 10 r/min,and fermentation time is 9 days.While Pennisetum sinese as the main base material is cultivated by solid fermentation,P .linteus mycelium grows well.The optimal fermentation conditions for P .linteus mycelium fermented with P .sinese are as follows:extraction temperature of 90 ℃,solid-liquid ratio of 1∶40,extraction time of 1.5 h, with 3 times-volume ethanol alcohol polysaccharide,under the above conditions,the polysaccharide yield reaches 12.06%,which has more mycelium polysaccharides than other cultivation methods.