CAJ | 학술논문

目的：通过上调miR－222，检测肝癌细胞增殖能力和侵袭能力的变化。方法采用载有miR－222超表达子的脂质体转染HepG2肝癌细胞系，使miR－222过表达；采用Western blot检测miR－222的靶蛋白PTEN的表达；MTT比色法检测转染前后HepG2细胞增殖能力的变化；Transwell试验检测转染前后细胞侵袭能力的变化。结果 Western blot 实验中转染组的PTEN蛋白表达量明显低于阴性对照组和空白对照组；在MTT实验中，转染组OD值较阴性对照组和空白对照组升高（P＜0．05）；在Transwell小室实验中，转染组的穿膜细胞数目较阴性对照组和空白对照组升高（ P＜0．05）。结论上调miR－222，HepG2细胞PTEN蛋白表达量明显降低，抑癌基因PTEN表达受抑制使肿瘤细胞的增殖能力和侵袭能力增强。
목적：통과상조miR－222，검측간암세포증식능력화침습능력적변화。방법채용재유miR－222초표체자적지질체전염HepG2간암세포계，사miR－222과표체；채용Western blot검측miR－222적파단백PTEN적표체；MTT비색법검측전염전후HepG2세포증식능력적변화；Transwell시험검측전염전후세포침습능력적변화。결과 Western blot 실험중전염조적PTEN단백표체량명현저우음성대조조화공백대조조；재MTT실험중，전염조OD치교음성대조조화공백대조조승고（P＜0．05）；재Transwell소실실험중，전염조적천막세포수목교음성대조조화공백대조조승고（ P＜0．05）。결론상조miR－222，HepG2세포PTEN단백표체량명현강저，억암기인PTEN표체수억제사종류세포적증식능력화침습능력증강。
Objective To detect the changes of cell invasion and proliferation in hepatocellular carcino -ma cell line HepG2 through the upregulation of miR -222.Methods The expression of miR-222 was up-reg-ulated by liposomal transfection .The expression of PTEN which is the targeting protein of miR -222 was detected by using Western Blot assay .The changes of proliferative capacity and invasive ability in HepG 2 cells after the upregulation of miR-222 were detected through MTT assay and Transwell assay .Results In Western Blot ex-periments,the expression of PTEN in transfection groups were lower than that in negative control groups and blank control groups.In MTT assay,the OD values in transfected group were higher than that in negative control group and blank control group(P<0.05).In transwell experiments,the number of penetrating cells in transfected group were higher than that in negative control group and blank control group (P<0.05).Conclusion Our results sug-gest that PTEN is significantly down regulated by the upregulation of miR -222.The proliferative capacity and inva-sive ability are raised in hepatocellular carcinoma cell line HepG 2 through the upregulation of miR-222.