CAJ | 학술논문

目的：探讨在荧光显微镜下，应用荧光素钠发出的荧光来确定胶质瘤边界，进而通过手术更彻底地切除胶质瘤。方法随机选择吉林大学第一医院收治的14例患者作为研究对象。患者术前依据其病史、体征、查体及影像检查，诊断为胶质瘤。术中应用荧光素钠所发出的黄色荧光来确定肿瘤边界，并切除肿瘤，根据荧光强度强弱不同，进行不同部位取材，病理诊断以确定荧光引导的边界是否准确，患者术后行MRI检查以明确肿瘤的切除情况，并观察患者术后神经功能情况。结果胶质瘤在荧光显微镜下能够被激发出黄荧光，而正常脑组织在荧光显微镜下不发光。术后病理结果显示黄色荧光区域内含有大量的胶质瘤细胞，淡黄色荧光区域内有少量的胶质瘤细胞。术后增强MRI扫描证实，应用荧光素钠能够更彻底地切除胶质瘤，患者术后功能障碍发生率减少。结论荧光素钠引导确定胶质瘤边界较肉眼评估更准确，降低肿瘤的复发率，减少正常脑组织损伤，提高患者术后生存质量。
목적：탐토재형광현미경하，응용형광소납발출적형광래학정효질류변계，진이통과수술경철저지절제효질류。방법수궤선택길림대학제일의원수치적14례환자작위연구대상。환자술전의거기병사、체정、사체급영상검사，진단위효질류。술중응용형광소납소발출적황색형광래학정종류변계，병절제종류，근거형광강도강약불동，진행불동부위취재，병리진단이학정형광인도적변계시부준학，환자술후행MRI검사이명학종류적절제정황，병관찰환자술후신경공능정황。결과효질류재형광현미경하능구피격발출황형광，이정상뇌조직재형광현미경하불발광。술후병리결과현시황색형광구역내함유대량적효질류세포，담황색형광구역내유소량적효질류세포。술후증강MRI소묘증실，응용형광소납능구경철저지절제효질류，환자술후공능장애발생솔감소。결론형광소납인도학정효질류변계교육안평고경준학，강저종류적복발솔，감소정상뇌조직손상，제고환자술후생존질량。
Objecitive Under the fluorescent microscope ,we used fluorescein sodium fluorescence to determine glioma boundary ,thus gliomas removed through surgery more thoroughly .Mtehods We randomly se-lected 14 patients who were admitted in the First Hospital of JiLin University as the research objects ,patients with glioma were diagnosed as glioma according to the physical signs ,physical examination a,nd imaging findings before surgery.Diagnosed with glioma,intraoperative application of fluorescein sodium yellow fluorescence was deter -mined the tumor boundary ,and removal of the tumor ,according to the fluorescence intensity strength is different . The pathological diagnosis was to determine the boundary of fluorescent was accurate .Postoperative examined MRI was performed in order to make clear the excision of the tumor ,and the neurological condition of postoperative was observed.Results Glioma could be inspired by yellow fluorescence under fluorescent microscope .The normal brain tissue was not light .Postoperative pathological results showed that the fluorescent yellow area contained a lot of glioma cells,pale yellow fluorescence area found a small amount of glioma cells .Postoperative enhanced MRI scan had confirmed that application of fluorescein sodium could be more thoroughly resection of glioma ,postopera-tive dysfunction was reduced .Conclusion This method is prior to tumor boundary observasion without fluoresent staining and reducing the recurrence of the tumor and reducing the normal brain tissue damage ,and therefore,im-proving the quality of postoperative survival of patients .