包头医学院学报
包頭醫學院學報
포두의학원학보
JOURNAL OF BAOTOU MEDICAL COLLEGE
2015年
6期
3-4
,共2页
慢性肾衰竭%红细胞%补体%受体%基因型
慢性腎衰竭%紅細胞%補體%受體%基因型
만성신쇠갈%홍세포%보체%수체%기인형
Chronic renal failure%Erythrocyte%Complement%Receptor%Genotype
目的：观察慢性肾衰竭（CRF）患者红细胞补体受体1型分子（erythrocyte receptor type 1，E－CR1）基因多态性以及 E－CR1表达和黏附活性的变化，探讨其临床意义。方法：选择100例慢性肾衰竭患者为CRF组，选取同期健康体检者50例为对照组。采用聚合酶链反应和Hind Ⅲ酶切技术测定红细胞CR1分子的基因型，采用酶联法测定红细胞 CR1的表达量，采用红细胞天然免疫黏附试验测定红细胞CR1分子黏附活性。结果：CRF 组红细胞 CR1基因多态性分布（ HH：55．0％、HL：31．0％、LL：14．0％）与对照组（ HH：70．0％、HL：24．0％、LL：6．0％）比较，差异无统计学意义（ P ＞0．05）；同种基因型CRF组和对照组组间比较，CRF组HH 型、HL型和LL型红细胞CR1表达量、黏附活性均明显低于对照组（ P ＜0．05）；CRF和对照组组内不同基因型之间比较，HH型红细胞 CR1表达量、黏附活性均明显高于HL型和LL型（ P ＜0．05）。结论：CRF的发生与红细胞CR1表达数量和黏附活性有关。
目的：觀察慢性腎衰竭（CRF）患者紅細胞補體受體1型分子（erythrocyte receptor type 1，E－CR1）基因多態性以及 E－CR1錶達和黏附活性的變化，探討其臨床意義。方法：選擇100例慢性腎衰竭患者為CRF組，選取同期健康體檢者50例為對照組。採用聚閤酶鏈反應和Hind Ⅲ酶切技術測定紅細胞CR1分子的基因型，採用酶聯法測定紅細胞 CR1的錶達量，採用紅細胞天然免疫黏附試驗測定紅細胞CR1分子黏附活性。結果：CRF 組紅細胞 CR1基因多態性分佈（ HH：55．0％、HL：31．0％、LL：14．0％）與對照組（ HH：70．0％、HL：24．0％、LL：6．0％）比較，差異無統計學意義（ P ＞0．05）；同種基因型CRF組和對照組組間比較，CRF組HH 型、HL型和LL型紅細胞CR1錶達量、黏附活性均明顯低于對照組（ P ＜0．05）；CRF和對照組組內不同基因型之間比較，HH型紅細胞 CR1錶達量、黏附活性均明顯高于HL型和LL型（ P ＜0．05）。結論：CRF的髮生與紅細胞CR1錶達數量和黏附活性有關。
목적：관찰만성신쇠갈（CRF）환자홍세포보체수체1형분자（erythrocyte receptor type 1，E－CR1）기인다태성이급 E－CR1표체화점부활성적변화，탐토기림상의의。방법：선택100례만성신쇠갈환자위CRF조，선취동기건강체검자50례위대조조。채용취합매련반응화Hind Ⅲ매절기술측정홍세포CR1분자적기인형，채용매련법측정홍세포 CR1적표체량，채용홍세포천연면역점부시험측정홍세포CR1분자점부활성。결과：CRF 조홍세포 CR1기인다태성분포（ HH：55．0％、HL：31．0％、LL：14．0％）여대조조（ HH：70．0％、HL：24．0％、LL：6．0％）비교，차이무통계학의의（ P ＞0．05）；동충기인형CRF조화대조조조간비교，CRF조HH 형、HL형화LL형홍세포CR1표체량、점부활성균명현저우대조조（ P ＜0．05）；CRF화대조조조내불동기인형지간비교，HH형홍세포 CR1표체량、점부활성균명현고우HL형화LL형（ P ＜0．05）。결론：CRF적발생여홍세포CR1표체수량화점부활성유관。
Objective:To investigate the change of gene polymorphism, expression and adhesive activity of erythrocyte complement receptor type 1 (E-CR1) in patients with chronic renal failure (CRF) and explore its clinical significance.Methods:100 cases were selected as the CRF group and 50 healthy subjects as the control group.Polymerase chain reaction ( PCR) and HindⅢrestriction enzyme digestion were adopted to de-termine genotype of E-CR1, enzyme immunoassay was used to detect the expression quantity of E -CR1 and erythrocyte innate immune adhesion test was made for the determination of adhesive activity E -CR1.Results:There was no significant difference in E -CR1gene polymorphism be-tween CRF group (HH:55.0 %、HL:31.0 %、LL:14.0 %) and the control group (HH:70.0 %、HL:24.0 %、LL:6.0 %)( P >0.05).When the same genotype was compared between CRF group and the control group, the expression quantity and adhesive activity of E -CR1 in HH, HL and LL type in the former group were significantly lower than those in the latter group( P <0.05).When the different genotypes were compared in CRF group and the control group respectively, the expression quantity and adhesive activity of E -CR1 in HH type were significantly higher than those in HL and LL type in the two groups( P <0.05).Conclusion:The occurrence of CRF is associated with the expression quantity and adhesive activity of E-CR1.
