解剖学杂志
解剖學雜誌
해부학잡지
CHINESE JOURNAL OF ANATOMY
2015年
3期
326-329
,共4页
热比亚木巴克%伊力哈木乃扎木%宋曼殳%多力坤·买买提玉素甫
熱比亞木巴剋%伊力哈木迺扎木%宋曼殳%多力坤·買買提玉素甫
열비아목파극%이력합목내찰목%송만수%다력곤·매매제옥소보
身高%细胞色素 P450c17α酶基因%多态性%维吾尔族
身高%細胞色素 P450c17α酶基因%多態性%維吾爾族
신고%세포색소 P450c17α매기인%다태성%유오이족
height%cytochrome P450cαgene%polymorphism%Uyghur nationality
目的：：探讨细胞色素 P450c17α酶基因(CYP17)多态性与新疆和田地区民丰县成人个体高矮是否具有相关性。方法：收集长期居住同一地区22例高身材组(男性14例,女性8例)和81例矮身材组(男性27例,女性54例)成人的血液标本并提取 DNA,设计引物,通过 PCR 扩增包括基因多态位点的片段,用限制性内切酶 MspA1I 进行酶切,产物在2%琼脂糖凝胶上电泳,确定 CYP17基因的3种基因型,即 A1/A1、A1/A2、A2/A2,并经测序证实。结果：在男女高矮个子组间A1/A2、A2/A2、A1/A1基因型频率的分布无差异；男性高矮个子组间 A1/A2、A2/A2、A1/A1基因型频率的分布呈显著性差异；A1/A1和 A2/A2基因型在高矮个子组间非条件 Logistic 回归分析,OR 值分别为1.128(95%CI 0.925~1.376)和0.778(95%CI 0.442~1.161)。女性高矮个子组间无差异。A1/A2、A2/A2、A1/A1基因型与所有样本生化指标间无差异,但身高间存在差异；维吾尔族与汉族人群基因型之间有差异,而与藏族人群间无差异。结论：CYP17基因 MspA1I 酶切位点基因多态性与维吾尔族男性成人身材高矮有相关性。CYP17基因 MspA1I 酶切位点多态性可能是影响维吾尔族成人个体身高差异的基因位点之一。
目的：：探討細胞色素 P450c17α酶基因(CYP17)多態性與新疆和田地區民豐縣成人箇體高矮是否具有相關性。方法：收集長期居住同一地區22例高身材組(男性14例,女性8例)和81例矮身材組(男性27例,女性54例)成人的血液標本併提取 DNA,設計引物,通過 PCR 擴增包括基因多態位點的片段,用限製性內切酶 MspA1I 進行酶切,產物在2%瓊脂糖凝膠上電泳,確定 CYP17基因的3種基因型,即 A1/A1、A1/A2、A2/A2,併經測序證實。結果：在男女高矮箇子組間A1/A2、A2/A2、A1/A1基因型頻率的分佈無差異；男性高矮箇子組間 A1/A2、A2/A2、A1/A1基因型頻率的分佈呈顯著性差異；A1/A1和 A2/A2基因型在高矮箇子組間非條件 Logistic 迴歸分析,OR 值分彆為1.128(95%CI 0.925~1.376)和0.778(95%CI 0.442~1.161)。女性高矮箇子組間無差異。A1/A2、A2/A2、A1/A1基因型與所有樣本生化指標間無差異,但身高間存在差異；維吾爾族與漢族人群基因型之間有差異,而與藏族人群間無差異。結論：CYP17基因 MspA1I 酶切位點基因多態性與維吾爾族男性成人身材高矮有相關性。CYP17基因 MspA1I 酶切位點多態性可能是影響維吾爾族成人箇體身高差異的基因位點之一。
목적：：탐토세포색소 P450c17α매기인(CYP17)다태성여신강화전지구민봉현성인개체고왜시부구유상관성。방법：수집장기거주동일지구22례고신재조(남성14례,녀성8례)화81례왜신재조(남성27례,녀성54례)성인적혈액표본병제취 DNA,설계인물,통과 PCR 확증포괄기인다태위점적편단,용한제성내절매 MspA1I 진행매절,산물재2%경지당응효상전영,학정 CYP17기인적3충기인형,즉 A1/A1、A1/A2、A2/A2,병경측서증실。결과：재남녀고왜개자조간A1/A2、A2/A2、A1/A1기인형빈솔적분포무차이；남성고왜개자조간 A1/A2、A2/A2、A1/A1기인형빈솔적분포정현저성차이；A1/A1화 A2/A2기인형재고왜개자조간비조건 Logistic 회귀분석,OR 치분별위1.128(95%CI 0.925~1.376)화0.778(95%CI 0.442~1.161)。녀성고왜개자조간무차이。A1/A2、A2/A2、A1/A1기인형여소유양본생화지표간무차이,단신고간존재차이；유오이족여한족인군기인형지간유차이,이여장족인군간무차이。결론：CYP17기인 MspA1I 매절위점기인다태성여유오이족남성성인신재고왜유상관성。CYP17기인 MspA1I 매절위점다태성가능시영향유오이족성인개체신고차이적기인위점지일。
Obj ective :To validate correlation of genetic polymorphism of CYP1 7 with height in the residents living in Mingfeng town,Hetian district of Xinjiang Uyghur autonomous region.Methods :The blood DNA samples were obtained from 22 adults (14 males and 8 females)as Tall Group and 81 adults (27 males and 54 females)as Short Group,respectively.Then, the three genotypes of CYP1 7 (A1/A1 ,A1/A2 and A2/A2 )were analyzed by using PCR and confirmed by sequencing. Results :No difference was found in genetic polymorphisms between two genders.However,the frequency distributions of three genotypes in two different male groups showed significant difference.And,the Logistic regression analysis showed that the OR values of A1/A1 and A2/A2 in Tall and Short groups of males were 1 .128 (95 % CI 0.925-1 .376)and 0.778(95 % CI 0.442-1 .1 6 1),respectively.Conversely,no significant differences were found in female individuals.The three genotypes of CYP1 7 did not correlate to parameters detected in present study excluding height.Moreover,the polymorphisms of CYP1 7 were obviously distinguished from the Han people,but similar to Tibetan people.Conclusion:The loci gene polymorphisms of CYP1 7 revealed by MspA1I enzyme digestion are correlated to height in Uygur males.The polymorphisms of CYP1 7 should be a potential candidate to influence the height of Uyghur adults.
