CAJ | 학술논문

目的：探讨布鲁杆菌病（布病）患者T淋巴细胞亚群的变化，为判断布病患者免疫功能变化提供依据。方法142例布病患者及45例健康体检人群（对照组）血样标本来自于黑龙江省农垦总局总医院检验科。采用流式细胞术方法测定CD3＋淋巴细胞百分比、CD19＋淋巴细胞百分比、CD3＋／CD4＋T淋巴细胞百分比、CD3＋／CD8＋T淋巴细胞百分比、CD4＋／CD8＋比值，同时进行统计学分析。结果与对照组比较布病组CD3＋％增多、CD19＋％减低、CD3＋／CD4＋％减低，CD3＋／CD8＋％增多，CD4＋／CD8＋比值减低，且P＜0．05变化有显著性意义。结论布病患者细胞免疫及体液免疫功能均发生变化。
목적：탐토포로간균병（포병）환자T림파세포아군적변화，위판단포병환자면역공능변화제공의거。방법142례포병환자급45례건강체검인군（대조조）혈양표본래자우흑룡강성농은총국총의원검험과。채용류식세포술방법측정CD3＋림파세포백분비、CD19＋림파세포백분비、CD3＋／CD4＋T림파세포백분비、CD3＋／CD8＋T림파세포백분비、CD4＋／CD8＋비치，동시진행통계학분석。결과여대조조비교포병조CD3＋％증다、CD19＋％감저、CD3＋／CD4＋％감저，CD3＋／CD8＋％증다，CD4＋／CD8＋비치감저，차P＜0．05변화유현저성의의。결론포병환자세포면역급체액면역공능균발생변화。
Objective To investigate the variation of lymphocyte subgroup in patients with Brucellosis , Provide the basis for judging the changes of immune function in patients with Brucellosis .Methods All the blood samples came from clinical laboratory of Heilongjiang Agricultural Reclamation Bureau General Hospital . Including 142 Brucella patients and 45 healthy people ( control group ) .Flow cytometric method was used to determine the percentage of CD3 +lymphocyte, the percentage of CD19 +lymphocyte, the percentage of CD3 +/CD4 +lymphocyte, the percentage of CD3 +/CD8 +lymphocyte and CD4 +/CD8 +ratio.Statistical analysis was carried on at the same time .Results CD3 +cells percentage in the Brucella group was increased compared with the control group , CD19 +, cells percentage in the Brucella group was decreased compared with the control group, CD3 +/CD4+ cells percentage in the Brucella group was decreased compared with the control group , CD3 +/CD8 +cells percentage in the Brucella group was increased compared with the control group , CD4 +/CD8 +ratio in the Brucella group was decreased compared with the control group ,and P<0.05 Significant changes. Conclusions Cellular immunity and humeral immunity functions are changed in different on the patients with Brucellosis .