CAJ | 학술논문

目的：探讨肺腺癌组织中EGFR19／21基因突变与Ki67，P53表达的相关性及临床意义。方法回顾性分析140例病理确诊为肺腺癌的患者，均经Taqman-ARMS法检测肿瘤组织EGFR19／21基因突变状况，并根据结果进行分组；应用免疫组化SP方法检测两组肿瘤组织中Ki67、P53蛋白的表达情况，对两组检测结果并结合其临床和病理资料应用SPSS 18．0进行统计学分析。结果140例肺腺癌病例中Ki67蛋白高表达率为（32／140，22．9％）；P53蛋白阳性表达率为（63／140，45％）；Ki67高表达与肿瘤大小、有否淋巴结转移、分化程度、P-TNM分期有显著相关（P＜0．001），与EGFR基因突变状况无关（P＝0．227， P＞0．05）。 P53表达与肿瘤大小、有否淋巴结转移、P-TNM分期有关（P＜0．05），与EGFR基因突变状况有关（P＝0．041，P＜0．05）。 EGFR基因突变与P53表达存在正相关性（r＝0．173，P＜0．05），与Ki67高表达不存在相关性（r ＝－0．102，P＞0．05）；P53和 Ki67表达间存在正相关性（r＝0．166，P＜0．05）。结论 EGFR基因突变，Ki67、P53蛋白表达在肺腺癌的发生、侵袭、转移起重要作用，联合检测对肺腺癌患者病理诊断、恶性程度判断、个体化治疗选择及预后评估有重要临床意义。
목적：탐토폐선암조직중EGFR19／21기인돌변여Ki67，P53표체적상관성급림상의의。방법회고성분석140례병리학진위폐선암적환자，균경Taqman-ARMS법검측종류조직EGFR19／21기인돌변상황，병근거결과진행분조；응용면역조화SP방법검측량조종류조직중Ki67、P53단백적표체정황，대량조검측결과병결합기림상화병리자료응용SPSS 18．0진행통계학분석。결과140례폐선암병례중Ki67단백고표체솔위（32／140，22．9％）；P53단백양성표체솔위（63／140，45％）；Ki67고표체여종류대소、유부림파결전이、분화정도、P-TNM분기유현저상관（P＜0．001），여EGFR기인돌변상황무관（P＝0．227， P＞0．05）。 P53표체여종류대소、유부림파결전이、P-TNM분기유관（P＜0．05），여EGFR기인돌변상황유관（P＝0．041，P＜0．05）。 EGFR기인돌변여P53표체존재정상관성（r＝0．173，P＜0．05），여Ki67고표체불존재상관성（r ＝－0．102，P＞0．05）；P53화 Ki67표체간존재정상관성（r＝0．166，P＜0．05）。결론 EGFR기인돌변，Ki67、P53단백표체재폐선암적발생、침습、전이기중요작용，연합검측대폐선암환자병리진단、악성정도판단、개체화치료선택급예후평고유중요림상의의。
Objective To research the relationship between EGFR 19/21 gene mutation in lung adenocarcinoma patients and lung cancer associated proteins of Ki 67 and P53 expressions and clinical significance .Methods Retrospective analysis of 140 cases of pathological diagnosed with lung adenocarcinoma patients specimens , the EGFR19/21 gene mutation in the tumor tissue used Taqman-ARMS assay, and divided into two groups according to the results .The expression of Ki67 and P53 proteins in tumor specimens of two groups used immunohistochemical SP method , The results of two groups of testing and the clinical and pathological data using SPSS 18.0 to statistical analysis.Results In the 140 cases of lung adenocarcinoma cases , Ki67 protein high expression rate (32/140,22.9%); P53 protein positive expression rate (63/140, 45%); Ki67 expression were significantly related with the tumor size , lymph node metastasis , differentiation , P-TNM stage (P<0.001), and no correlated with the EGFR mutation status (P=0.227, P>0.227).P53 expression were related with the tumor size, lymph node metastasis and P-TNM staging ( P <0.05), associated with EGFR mutation situation (P=0.041, P<0.05).EGFR gene mutation and P53 positive correlation (r=0.173, P<0.05), EGFR gene mutation and Ki67 doesn't exist correlation (r=0.102, P>0.102); Positive correlations between P53 and Ki67 expressions (r =0.166, P <0.05).Conclusions The EGFR gene mutation, the expression of Ki67 and P53 proteins played an important role in lung adenocarcinoma carcinogenesis and invasion and metastasis , the joint detection of lung adenocarcinoma patients with pathologic diagnosis , judging malignant degree, individualized treatment choice and prognosis assessment has important clinical significance .