CAJ | 학술논문

目的：探讨血浆甘露聚糖结合凝集素（MBL）相关丝氨酸蛋白酶2（MASP2）水平在儿童上呼吸道感染（上感）中的意义。方法取103例上感患儿和35例健康体检儿童作为研究对象，测定其血浆MASP2和C反应蛋白（CRP）浓度并进行白细胞计数（WBC）。根据CRP和WBC值、感染不同时期及有无治疗，上感儿童分为CRP升高组（n=48）与正常组（n=54）、WBC升高组（n=61）与正常组（n=40）、感染早期未用药组（n=68）与感染中后期已用药组（n=35），对各组资料进行统计学分析。结果上感组MASP2浓度显著高于对照组（P<0.001），CRP升高组血浆MASP2浓度与CRP值正相关（r=0.310，P<0.05），WBC升高组MASP2水平与WBC值显著正相关（r=0.392，P<0.01），感染早期未用药组MASP2浓度显著高于感染中后期已用药组（P<0.01）， MASP2、CRP、MBL2基因具有共同的转录因子HNF-4α结合位点。结论 MASP2蛋白可能为急性期蛋白，血浆MASP2水平可作为辅助诊断小儿上感的一个参考指标。
목적：탐토혈장감로취당결합응집소（MBL）상관사안산단백매2（MASP2）수평재인동상호흡도감염（상감）중적의의。방법취103례상감환인화35례건강체검인동작위연구대상，측정기혈장MASP2화C반응단백（CRP）농도병진행백세포계수（WBC）。근거CRP화WBC치、감염불동시기급유무치료，상감인동분위CRP승고조（n=48）여정상조（n=54）、WBC승고조（n=61）여정상조（n=40）、감염조기미용약조（n=68）여감염중후기이용약조（n=35），대각조자료진행통계학분석。결과상감조MASP2농도현저고우대조조（P<0.001），CRP승고조혈장MASP2농도여CRP치정상관（r=0.310，P<0.05），WBC승고조MASP2수평여WBC치현저정상관（r=0.392，P<0.01），감염조기미용약조MASP2농도현저고우감염중후기이용약조（P<0.01）， MASP2、CRP、MBL2기인구유공동적전록인자HNF-4α결합위점。결론 MASP2단백가능위급성기단백，혈장MASP2수평가작위보조진단소인상감적일개삼고지표。
Absteact:Objective To explore the significance of plasma levels of mannan-binding lectin (MBL)-associated serine protease 2 (MASP2) in children with upper respiratory tract infection (URTI). Methods A total of 103 children with URTI and 35 healthy children were examined for plasma levels of MASP2 and C-reactive protein (CRP). According to CRP levels, white blood cell count (WBC), stage of infection, and administration of treatments, the children with URTI were divided into the elevated CRP group (n=48) and the normal CRP group (n=54), elevated WBC group (n=61) and normal WBC group (n=40), the early stage of infection without treatment group (n=68) and mid-late stage of infection with treatment group (n=35). Results Plasma MASP2 levels was significantly higher in URTI group than in the healthy control group (P<0.001) and showed a close correlation with age (r=0.302, P<0.01). Plasma MASP2 level was significantly correlated with CRP level in elevated CRP group (r=0.310, P<0.05) but not in normal CRP group (P>0.05), correlated with WBC in elevated WBC group (r=0.392, P<0.01) but not in normal WBC group (P>0.05), and was significantly higher in early stage infection without treatment group than in mid-late stage of infection with treatment group (P<0.01). MASP2, MBL2 and CRP genes had a common binding site for the transcription factor HNF-4α. Conclusion MASP2 may be an acute-phase protein, and its plasma level might serve as a new reference index in the diagnosis of URTI in children.