中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2015年
17期
144-146
,共3页
脑脊液%LA%IL8%化脓性脑膜炎
腦脊液%LA%IL8%化膿性腦膜炎
뇌척액%LA%IL8%화농성뇌막염
Cerebrospinal fluid%Lactic acid%Interleukin-8%Purulent meningitis
目的：通过检测化脓性脑膜炎（PM）患儿脑脊液中乳酸（LA）、白细胞介素-8（IL-8）的浓度变化，探讨其在化脓性脑膜炎的变化及临床意义。方法：采用COBAS INTEGRA 400PLUS全自动生化分析仪及双抗体夹心酶联免疫吸附试验（ELISA）对60例化脓性脑膜炎患者、42例病毒性脑炎（VE）患者、54例对照组儿童急性期及恢复期脑脊液中乳酸（LA）和IL-8变化进行检测。观察LA、IL-8与中性粒细胞的相关性。结果：急性期PM组脑脊液中LA和IL-8分别为（4.71±2.43）mmol/L和（261.02±58.14）ng/L明显高于VE组（2.10±0.44）mmol/L和（94.86±41.27）ng/L，对照组（1.95±0.51）mmol/L和（84.65±19.7）ng/L，三组比较差异有统计学意义（P<0.01）；恢复期PM组LA和IL-8分别为（3.23±2.11）mmol/L和（204.24±48.53）ng/L较急性期明显下降，差异有统计学意义（P<0.01）。脑脊液中LA和IL-8水平及中性粒细胞相关性分析显示：PM组LA及IL-8呈正相关（r=0.482， P<0.01），PM组IL-8与中性粒细胞呈正相关（r=0.397，P<0.01）；VE组IL-8与中性粒细胞无相关性（r=0.299， P>0.05）。结论：乳酸、IL-8参与化脓性脑膜炎局部炎性应答，提示脑脊液中IL-8及LA的检测可作为化脓性脑膜炎诊断及病情变化判断的一项参考指标。
目的：通過檢測化膿性腦膜炎（PM）患兒腦脊液中乳痠（LA）、白細胞介素-8（IL-8）的濃度變化，探討其在化膿性腦膜炎的變化及臨床意義。方法：採用COBAS INTEGRA 400PLUS全自動生化分析儀及雙抗體夾心酶聯免疫吸附試驗（ELISA）對60例化膿性腦膜炎患者、42例病毒性腦炎（VE）患者、54例對照組兒童急性期及恢複期腦脊液中乳痠（LA）和IL-8變化進行檢測。觀察LA、IL-8與中性粒細胞的相關性。結果：急性期PM組腦脊液中LA和IL-8分彆為（4.71±2.43）mmol/L和（261.02±58.14）ng/L明顯高于VE組（2.10±0.44）mmol/L和（94.86±41.27）ng/L，對照組（1.95±0.51）mmol/L和（84.65±19.7）ng/L，三組比較差異有統計學意義（P<0.01）；恢複期PM組LA和IL-8分彆為（3.23±2.11）mmol/L和（204.24±48.53）ng/L較急性期明顯下降，差異有統計學意義（P<0.01）。腦脊液中LA和IL-8水平及中性粒細胞相關性分析顯示：PM組LA及IL-8呈正相關（r=0.482， P<0.01），PM組IL-8與中性粒細胞呈正相關（r=0.397，P<0.01）；VE組IL-8與中性粒細胞無相關性（r=0.299， P>0.05）。結論：乳痠、IL-8參與化膿性腦膜炎跼部炎性應答，提示腦脊液中IL-8及LA的檢測可作為化膿性腦膜炎診斷及病情變化判斷的一項參攷指標。
목적：통과검측화농성뇌막염（PM）환인뇌척액중유산（LA）、백세포개소-8（IL-8）적농도변화，탐토기재화농성뇌막염적변화급림상의의。방법：채용COBAS INTEGRA 400PLUS전자동생화분석의급쌍항체협심매련면역흡부시험（ELISA）대60례화농성뇌막염환자、42례병독성뇌염（VE）환자、54례대조조인동급성기급회복기뇌척액중유산（LA）화IL-8변화진행검측。관찰LA、IL-8여중성립세포적상관성。결과：급성기PM조뇌척액중LA화IL-8분별위（4.71±2.43）mmol/L화（261.02±58.14）ng/L명현고우VE조（2.10±0.44）mmol/L화（94.86±41.27）ng/L，대조조（1.95±0.51）mmol/L화（84.65±19.7）ng/L，삼조비교차이유통계학의의（P<0.01）；회복기PM조LA화IL-8분별위（3.23±2.11）mmol/L화（204.24±48.53）ng/L교급성기명현하강，차이유통계학의의（P<0.01）。뇌척액중LA화IL-8수평급중성립세포상관성분석현시：PM조LA급IL-8정정상관（r=0.482， P<0.01），PM조IL-8여중성립세포정정상관（r=0.397，P<0.01）；VE조IL-8여중성립세포무상관성（r=0.299， P>0.05）。결론：유산、IL-8삼여화농성뇌막염국부염성응답，제시뇌척액중IL-8급LA적검측가작위화농성뇌막염진단급병정변화판단적일항삼고지표。
Objective:To detect the purulent meningitis (PM) lactic acid in cerebrospinal fluid in children with LA, interleukin-8 (IL-8) concentration changes,explore their changes in purulent meningitis and its clinical significance.Method:60 cases of purulent meningitis as the PM group,42 cases with viral encephalitis as the VE group,54 cases with no central nervous system diseases as the control group,all of children acute and recovery phase of lactic acid in cerebrospinal fluid (LA) and IL-8 were selected and detected the change by using COBAS INTEGRA 400PLUS automatic biochemical analyzer and double antibody sand wich enzyme linked immunosorbent assay (ELISA).The correlation between LA and IL-8 were observed with neutrophils. Result:In PM group,LA and IL-8 in cerebrospinal fluid were (4.71±2.43)mmol/L and (261.02±58.14)ng/L higher than that in VE group (2.10±0.44)mmol/L and (94.86±41.27)ng/L,control group (1.95±0.51)mmol/L and (84.65±19.7)ng/L, the differences were statistically significant (P<0.01);recovery PM group LA and IL-8 were (3.23±2.11) mmol/L and (204.24±48.53)ng/L decreased significantly which compared with acute period,the differences were statistically significant (P<0.01).Analysis of cerebrospinal fluid LA and IL-8 levels and neutrophil associated display:PM group LA was positively related to IL-8(r=0.482,P<0.01); PM group IL-8 was positively associated with neutrophil(r=0.397,P<0.01); there was no significant correlation in VE group between IL-8 and neutrophils(r=0.299,P>0.05).Conclusion:Lactic acid and IL-8 participates in the local inflammatory response of purulent meningitis.Suggest a reference index for detection of IL-8 and LA in the cerebrospinal fluid as purulent meningitis diagnosis and illness change judgment.
