实用检验医师杂志
實用檢驗醫師雜誌
실용검험의사잡지
Chinese Journal of Laboratory Pathologist
2015年
2期
90-93
,共4页
荣亮%候慧%郑森元%李可
榮亮%候慧%鄭森元%李可
영량%후혜%정삼원%리가
经内镜逆行性胰胆管造影术%ERCP术后胰腺炎%血清淀粉酶%胰蛋白酶原-2%白细胞介素-10%血小板活化因子
經內鏡逆行性胰膽管造影術%ERCP術後胰腺炎%血清澱粉酶%胰蛋白酶原-2%白細胞介素-10%血小闆活化因子
경내경역행성이담관조영술%ERCP술후이선염%혈청정분매%이단백매원-2%백세포개소-10%혈소판활화인자
Endoscopic retrograde cholangiopancreatography%Post-ERCP pancreatitis%Serum amy-lase%Trypsinogen-2%IL-10%Platelet activating factor
目的：探讨血清淀粉酶、胰蛋白酶原－2、白介素－10（interleukin－10， IL－10）和血小板活化因子（platelet activating factor， PAF）在经内镜逆行性胰胆管造影术（endoscopic retrograde cholangiopan-creatography， ERCP）术后胰腺炎（post－ERCP pancreatitis， PEP）患者中的表达及其联合检测在PEP中的诊断价值。方法收集我院2012年8月至2013年11月的ERCP患者120例，分为对照组（60例）和PEP组（60例），采用强生Vitros－350全自动干化学生化分析仪检测所有患者术前、术后4 h及术后24 h血清淀粉酶、胰蛋白酶原－2、IL－10和PAF水平，并对结果进行统计学分析。结果 PEP患者术后4 h和术后24 h血清淀粉酶、胰蛋白酶原－2、IL－10和PAF水平均显著高于术前及对照组术后4 h和术后24 h，差异均有统计学意义（P均＜0.05）。 PEP组患者血清淀粉酶水平在术后24 h最高，胰蛋白酶原－2、IL－10和PAF水平均为术后4 h最高。4项指标联合检测的灵敏度、特异性和准确度分别为95.0％、91.7％和85.0％，均高于各项指标单独检测。结论血清淀粉酶、胰蛋白酶原－2、IL－10和PAF水平单项检测均可用于PEP的诊断，联合检测可提高对PEP的诊断价值。
目的：探討血清澱粉酶、胰蛋白酶原－2、白介素－10（interleukin－10， IL－10）和血小闆活化因子（platelet activating factor， PAF）在經內鏡逆行性胰膽管造影術（endoscopic retrograde cholangiopan-creatography， ERCP）術後胰腺炎（post－ERCP pancreatitis， PEP）患者中的錶達及其聯閤檢測在PEP中的診斷價值。方法收集我院2012年8月至2013年11月的ERCP患者120例，分為對照組（60例）和PEP組（60例），採用彊生Vitros－350全自動榦化學生化分析儀檢測所有患者術前、術後4 h及術後24 h血清澱粉酶、胰蛋白酶原－2、IL－10和PAF水平，併對結果進行統計學分析。結果 PEP患者術後4 h和術後24 h血清澱粉酶、胰蛋白酶原－2、IL－10和PAF水平均顯著高于術前及對照組術後4 h和術後24 h，差異均有統計學意義（P均＜0.05）。 PEP組患者血清澱粉酶水平在術後24 h最高，胰蛋白酶原－2、IL－10和PAF水平均為術後4 h最高。4項指標聯閤檢測的靈敏度、特異性和準確度分彆為95.0％、91.7％和85.0％，均高于各項指標單獨檢測。結論血清澱粉酶、胰蛋白酶原－2、IL－10和PAF水平單項檢測均可用于PEP的診斷，聯閤檢測可提高對PEP的診斷價值。
목적：탐토혈청정분매、이단백매원－2、백개소－10（interleukin－10， IL－10）화혈소판활화인자（platelet activating factor， PAF）재경내경역행성이담관조영술（endoscopic retrograde cholangiopan-creatography， ERCP）술후이선염（post－ERCP pancreatitis， PEP）환자중적표체급기연합검측재PEP중적진단개치。방법수집아원2012년8월지2013년11월적ERCP환자120례，분위대조조（60례）화PEP조（60례），채용강생Vitros－350전자동간화학생화분석의검측소유환자술전、술후4 h급술후24 h혈청정분매、이단백매원－2、IL－10화PAF수평，병대결과진행통계학분석。결과 PEP환자술후4 h화술후24 h혈청정분매、이단백매원－2、IL－10화PAF수평균현저고우술전급대조조술후4 h화술후24 h，차이균유통계학의의（P균＜0.05）。 PEP조환자혈청정분매수평재술후24 h최고，이단백매원－2、IL－10화PAF수평균위술후4 h최고。4항지표연합검측적령민도、특이성화준학도분별위95.0％、91.7％화85.0％，균고우각항지표단독검측。결론혈청정분매、이단백매원－2、IL－10화PAF수평단항검측균가용우PEP적진단，연합검측가제고대PEP적진단개치。
Objective To evaluate the expression of serum amylase, trypsinogen-2, interleukin-10 (IL-10) and platelet activating factor (PAF) in post-ERCP pancreatitis (PEP) patients, and their diagnostic value of combined detection in the diagnosis of PEP. Methods 120 cases of ERCP patients were divided into control group(60 cases) and PEP group(60 cases) in our hospital from August 2012 to November 2013. Serum amylase, trypsinogen-2, IL-10 and PAF levels before operation, 4 h after surgery and postoperative 24 h in the two groups were detected by Johnson&johnson Vitros-350 automatic chemical and biochemical analyzer , and the test results were statistically analyzed. Results In patients with PEP, 4 h after surgery and postopera-tive 24 h serum amylase, trypsinogen-2, IL-10 and PAF levels were all higher than those of before operation and 4 h after surgery and postoperative 24 h in control group, and the differences all had statistical significance (Pall<0.05). The highest level of serum amylase in PEP group was postoperative 24 h, and the highest levels of trypsinogen-2, IL-10 and PAF were 4 h after surgery. The sensitivity, specificity and accuracy of combined detection were 95.0%, 91.7% and 85.0%, respectively, which all higher than indicators single detection. Conclusion Serum amylase, trypsinogen-2, IL-10 and PAF levels individual detection has clinical signifi-cance in PEP disease, and the combined detection of serum amylase, trypsinogen-2, IL-10 and PAF levels can improve the early diagnostic value of PEP.
