CAJ | 학술논문

以体外非酶糖基化反应抑制率为考察指标，对猴头菌发酵液中抑制非酶糖基化反应的高活性物质进行分离并纯化，通过发酵液浓缩、二步醇沉、提取物冷冻干燥、C18层析柱等步骤进行分离，获得1种对非酶糖基化反应具有良好抑制作用的物质组分HE35，经液相色谱质谱（LC MS）分析后发现，HE35的相对分子质量为294，2 mg／mL的HE35对体外非酶糖基化抑制率达到92?17％，半抑制质量浓度IC50为0?65 mg／mL。初步分析猴头菌发酵液中可能存在一种以上的活性物质，对体外非酶糖基化反应具有抑制作用。
이체외비매당기화반응억제솔위고찰지표，대후두균발효액중억제비매당기화반응적고활성물질진행분리병순화，통과발효액농축、이보순침、제취물냉동간조、C18층석주등보취진행분리，획득1충대비매당기화반응구유량호억제작용적물질조분HE35，경액상색보질보（LC MS）분석후발현，HE35적상대분자질량위294，2 mg／mL적HE35대체외비매당기화억제솔체도92?17％，반억제질량농도IC50위0?65 mg／mL。초보분석후두균발효액중가능존재일충이상적활성물질，대체외비매당기화반응구유억제작용。
The bioactive component inhibited non?enzymatic glycosylation ( NEG ) reaction in Hericium erinaceus culture broth was purified by detecting the inhibitory rate against NEG reaction?The purified procedure included the fermentation broth concentration, two?step ethanol precipitation, lyophilization of extracts and C18 chromatography,and the high inhibitory component of HE35 was obtained. Analyzing by liquid chromatography?mass spectrometry ( LC?MS ) , the relative molecular mass was 294.In vitro experiment,the inhibition rate of HE35 at 2 mg/mL against NEG reaction was 92?17% and its IC50 was 0?65 mg/mL.The purification results indicate that more than one component in H?erinaceus culture broth possess the inhibitory activity to NEG reaction.