CAJ | 학술논문

为研究优化毕赤酵母工程菌H311产植酸酶的发酵条件，采用单因素试验和L18（37）正交试验考察不同工艺条件对产酶活性的影响。结果表明：影响重组毕赤酵母产植酸酶的因素重要性从大到小依次为诱导时间、甲醇添加量、装液量、初始诱导pH、生长时间、接种量和初始生长pH，产酶最佳条件为接种量3％（体积分数）、装液量20 mL（250 mL摇瓶）、生长时间20 h，诱导时间120 h、甲醇添加量1?5％（体积分数）、生长pH 6?0、诱导pH 5?0，在此条件下进行诱导表达，植酸酶的比酶活可达334 U／mL。
위연구우화필적효모공정균H311산식산매적발효조건，채용단인소시험화L18（37）정교시험고찰불동공예조건대산매활성적영향。결과표명：영향중조필적효모산식산매적인소중요성종대도소의차위유도시간、갑순첨가량、장액량、초시유도pH、생장시간、접충량화초시생장pH，산매최가조건위접충량3％（체적분수）、장액량20 mL（250 mL요병）、생장시간20 h，유도시간120 h、갑순첨가량1?5％（체적분수）、생장pH 6?0、유도pH 5?0，재차조건하진행유도표체，식산매적비매활가체334 U／mL。
To optimize the condition for fermentation of phytase production by recombinant Pichia pastoris H311. The effects of inoculum,liquid volume,growth time,induction time,methanol concentration,initial growth pH and initial induction pH on expression of phytase in recombinant P?pastoris H311were studied by single factor experiment and L18 ( 37 ) orthogonal experiment. Recombinant phytase was then characterized. The influencing factors on the expression of phytase were in the following order:induction time,methanol concentration, liquid volume, the initial induction pH value, growth time, inoculum and initial growth pH value. The optimal condition was with 3%(v/v) inoculum,20 mL liquid volume(250 mL flask) ,20 h growth time,120 h induction time,1?5%( v/v) methanol concentration,initial growth pH 6?0 value and initial induction pH 5?0. Under the optimal condition,the recombinant phytase reached an activity of 334 U/mL.