CAJ | 학술논문

米根霉交替呼吸强度与富马酸合成之间存在重要关联。以米根霉F 14为出发菌株，通过常压室温等离子体（ ARTP ）离子诱变技术，筛选出1株交替呼吸强度增强的突变菌株S 1。该菌株的交替呼吸初始强度是出发菌株F 14的3?5倍，然而，该菌株富马酸积累量（28 g／L）却远低于出发菌株（42 g／L）。进一步考察突变株S 1和出发株F 14菌株在发酵过程中交替呼吸及富马酸生产强度，总呼吸变化和还原力NADH／NAD＋的变化。结果表明：突变株在富马酸发酵初始阶段，过高的交替呼吸强度反而降低了富马酸的生产速率，并导致了发酵后期菌体的早衰现象。交替呼吸强度与发酵进程存在适配性，只有当适配性达到最佳状态时，才有利于产物的高效积累。
미근매교체호흡강도여부마산합성지간존재중요관련。이미근매F 14위출발균주，통과상압실온등리자체（ ARTP ）리자유변기술，사선출1주교체호흡강도증강적돌변균주S 1。해균주적교체호흡초시강도시출발균주F 14적3?5배，연이，해균주부마산적루량（28 g／L）각원저우출발균주（42 g／L）。진일보고찰돌변주S 1화출발주F 14균주재발효과정중교체호흡급부마산생산강도，총호흡변화화환원력NADH／NAD＋적변화。결과표명：돌변주재부마산발효초시계단，과고적교체호흡강도반이강저료부마산적생산속솔，병도치료발효후기균체적조쇠현상。교체호흡강도여발효진정존재괄배성，지유당괄배성체도최가상태시，재유리우산물적고효적루。
During fumaric acid fermentation, alternative respiration is associated with fumaric acid productivity in Rhizopus oryzae?S?1 mutant with higher activity of alternative respiration was screened by atmospheric and room temperature plasma ( ARTP ) ion mutagenesis using Rhizopus oryzae F?14 as the original strain. The activity of alternative respiration in S?1mutant is 3?5?fold than that of F?14. However, fumaric acid production ( 28 g/L ) was much lower than that of the original strain ( 42 g/L ) . The relationship between alternative respiration ratio and fumaric acid productivity, the change of total respiration activity and NADH/NAD+of S?1 and F?14 in the fermentation process were further studied. At the initial fermentation stage higher activity of alternative respiration in S?1 mutant reduced the fumaric acid productivity,and ultimately led to the premature aging phenomenon in the fermentation. It means that the activity of alternative respiration should adapt the fumaric acid productivity.