CAJ | 학술논문

采用高效液相色谱技术研究了苦皮藤素Ⅴ在粘虫Mythimna separata和小地老虎Agrotis ypsilon幼虫中的穿透及代谢。结果表明：苦皮藤素V均不能从粘虫和小地老虎幼虫的体壁穿透到血腔或从血腔穿透到中肠，但很容易从中肠穿透到血腔，且穿透速率无差异；苦皮藤素V在小地老虎幼虫体内的代谢解毒速率远大于其在粘虫幼虫体内的代谢速率，其半衰期分别为5.5和13.1 h。本研究结果表明，苦皮藤素V对粘虫和小地老虎幼虫的选择毒杀作用与药物的穿透能力无关，其在试虫体内的解毒代谢差异才是其对昆虫具有选择作用的机理之一。
채용고효액상색보기술연구료고피등소Ⅴ재점충Mythimna separata화소지로호Agrotis ypsilon유충중적천투급대사。결과표명：고피등소V균불능종점충화소지로호유충적체벽천투도혈강혹종혈강천투도중장，단흔용역종중장천투도혈강，차천투속솔무차이；고피등소V재소지로호유충체내적대사해독속솔원대우기재점충유충체내적대사속솔，기반쇠기분별위5.5화13.1 h。본연구결과표명，고피등소V대점충화소지로호유충적선택독살작용여약물적천투능력무관，기재시충체내적해독대사차이재시기대곤충구유선택작용적궤리지일。
Penetration and metabolism of celangulin V in the larvae of Mythimna separata and Agrotis ypsilon were studied using high performance liquid chromatography. The results showed that celangulin V can not penetrate into the haemolymph from the body wall or penetrate into the midgut from the haemolymph in both of larvae. Whereas,celangulin V with the same rate of penetration is easily penetrate into the haemolymph from the midgut in the case of both larvae. The metabolic rate of celangulin V in the larvae of A. ypsilon is greater than that in the larvae of M. separata and the half-life of celangulin V was 5. 5 h and 13. 1 h in the larvae of A. ypsilon and M. separata,respectively. The results indicate that the penetration was not involved in the selective toxicity of celangulin V,whereas, detoxification metabolism in the larvae of A. ypsilon and M. separata was one of the mechanisms of this selective toxicity.