CAJ | 학술논문

从垂序商陆中筛选具有抗烟草花叶病毒（ Tobacco mosaic virus，TMV）活性的小分子化合物，为开发高效、低毒和环境友好的天然抗病毒剂提供依据。通过现代化分离、纯化技术，采用活性追踪的方法，对采自于贵州省的垂序商陆中的化学成分进行抗TMV活性逐级筛选，获得了两个抗TMV活性较强的三萜皂苷类化合物—商陆皂苷丁和商陆皂苷H，利用光谱解析手段确定了化合物的结构。生物活性测定结果表明：商陆皂苷丁和商陆皂苷H均具有一定的活体钝化与抑制病毒增殖作用，其中活体钝化及病毒增殖抑制作用均强于对照药物宁南霉素。该研究首次发现垂序商陆中的三萜皂苷类化合物具有抗TMV的活性，为下一步系统研究该种植物提供了科学依据。
종수서상륙중사선구유항연초화협병독（ Tobacco mosaic virus，TMV）활성적소분자화합물，위개발고효、저독화배경우호적천연항병독제제공의거。통과현대화분리、순화기술，채용활성추종적방법，대채자우귀주성적수서상륙중적화학성분진행항TMV활성축급사선，획득료량개항TMV활성교강적삼첩조감류화합물—상륙조감정화상륙조감H，이용광보해석수단학정료화합물적결구。생물활성측정결과표명：상륙조감정화상륙조감H균구유일정적활체둔화여억제병독증식작용，기중활체둔화급병독증식억제작용균강우대조약물저남매소。해연구수차발현수서상륙중적삼첩조감류화합물구유항TMV적활성，위하일보계통연구해충식물제공료과학의거。
Small molecules isolated from Phytolacca americana L. grown in Guizhou province were evaluated for their anti tobacco mosaic virus( TMV)properties. Two triterpenoidsaponin compounds, eseulentoside D and esculentoside H showed higher activities than ningnanmycin AS. Their structures were determined by 1 H NMR and IR analysis. Notably,it is the first time that triterpenoidsaponin compounds from P. americana was found with anti-TMV activity. This research provides useful information for the systemically study of P. americana. It is also high valuable for developing efficient, low toxicity,environmental friendly antiviral agents.