CAJ | 학술논문

本研究对西藏地区豆科牧草青贮饲料中的乳酸菌进行分离、鉴定，以期得到优良的乳酸菌菌株。以苜蓿和箭筈豌豆青贮饲料为试验材料，共分离得到6株同型发酵乳酸菌，经传统微生物培养和16S rRNA 序列分析，3株（LCG9，CG35和 AG11）为戊糖片球菌，2株（LCG3和 LAG1）为植物乳杆菌，1株（LA3）为弯曲乳杆菌；除 AG11和LAG1在5℃，LCG9和 AG11在 pH 3．0和8．0生长微弱外，其余乳酸菌菌株均在5～20℃，pH 3．0～8．0及3．0％和6．5％ NaCl 培养液中生长良好。从耐低温特性、产酸能力和发酵速率3个指标考虑，植物乳杆菌 LCG3最适宜用于西藏青贮饲料生产的乳酸菌菌种。
본연구대서장지구두과목초청저사료중적유산균진행분리、감정，이기득도우량적유산균균주。이목숙화전괄완두청저사료위시험재료，공분리득도6주동형발효유산균，경전통미생물배양화16S rRNA 서렬분석，3주（LCG9，CG35화 AG11）위무당편구균，2주（LCG3화 LAG1）위식물유간균，1주（LA3）위만곡유간균；제 AG11화LAG1재5℃，LCG9화 AG11재 pH 3．0화8．0생장미약외，기여유산균균주균재5～20℃，pH 3．0～8．0급3．0％화6．5％ NaCl 배양액중생장량호。종내저온특성、산산능력화발효속솔3개지표고필，식물유간균 LCG3최괄의용우서장청저사료생산적유산균균충。
The objectives of this study were to identify lactic acid bacteria with desirable attributes by isolating and identifying lactic acid bacteria (LAB)from legume silages in Tibetan Plateau.A total of 6 homofermentia-tive lactic acid bacteria were isolated from alfalfa (Medicago sativa )and common vetch (Vicia sativa )silage. Strains LCG9,CG35 and AG11 were identified as Pediococcus pentosaceus ,strains LCG3 and LAG1 as Lacto-bacillus plantarum and strain LA3 as Lactobacillus curvatu .Conventional microbial identification methods and 16S rRNA sequencing analyses were used to identify different strains.The selected strains were grown in a range of conditions;temperature 5 -20℃,pH 3.0 -8.0 and NaCl 3.0% and 6.5%.LCG9 and AG11 grew weakly at 5℃ while LCG9 and AG11 were inactive at pH 3.0 and pH 8.0.LCG3 was identified as the best strain to use as a silage additive in Tibet due to its unique tolerance of low temperature,high lactic acid produc-tion and rapid reproductive rate.