CAJ | 학술논문

土壤微生物是土壤生态系统的重要组成部分，在生态系统物质循环和能量转化中占有重要的地位。以祁连山中段不同海拔的4种植物群落，即垫状植被（cushion plants，CP）、高寒草甸（alpine meadow，AM）、沼泽化草甸（swamp meadow，SM）和高寒灌丛（alpine shrub，AS）作为实验样地，采用 BIOLOG 技术，探讨了土壤微生物群落多样性在不用海拔梯度形成的植被条件下的变化特征。结果表明，反映微生物活性的平均颜色变化率（average well color development，AWCD）的大小顺序为：沼泽化草甸＞高寒草甸＞高寒灌丛＞垫状植被，沼泽化草甸土壤微生物群落代谢活性最高；高寒草甸和沼泽化草甸土壤微生物群落碳源利用模式相似，土壤微生物群落的Shannon－Wiener 物种丰富度指数（H ）与土壤有机碳和全氮显著相关（P ＜0．05），Simpson 优势度指数（D ）和 Mc-Intosh 指数（U ）与土壤全氮显著相关（P ＜0．05）；冗余分析表明，土壤有机碳、pH 和全氮可能是土壤微生物利用碳源的控制因子。研究结果为进一步探讨高寒草甸与土壤微生物之间的关系奠定了基础。
토양미생물시토양생태계통적중요조성부분，재생태계통물질순배화능량전화중점유중요적지위。이기련산중단불동해발적4충식물군락，즉점상식피（cushion plants，CP）、고한초전（alpine meadow，AM）、소택화초전（swamp meadow，SM）화고한관총（alpine shrub，AS）작위실험양지，채용 BIOLOG 기술，탐토료토양미생물군락다양성재불용해발제도형성적식피조건하적변화특정。결과표명，반영미생물활성적평균안색변화솔（average well color development，AWCD）적대소순서위：소택화초전＞고한초전＞고한관총＞점상식피，소택화초전토양미생물군락대사활성최고；고한초전화소택화초전토양미생물군락탄원이용모식상사，토양미생물군락적Shannon－Wiener 물충봉부도지수（H ）여토양유궤탄화전담현저상관（P ＜0．05），Simpson 우세도지수（D ）화 Mc-Intosh 지수（U ）여토양전담현저상관（P ＜0．05）；용여분석표명，토양유궤탄、pH 화전담가능시토양미생물이용탄원적공제인자。연구결과위진일보탐토고한초전여토양미생물지간적관계전정료기출。
Soil microbes are an important component of soil ecosystem playing a key role in nutrient and energy cycling.The variation of soil microbial community diversity under cushion plants,alpine meadow,swamp meadow and alpine shrub along an elevation gradient in mid the altitude zone of the Qilian Mountains was meas-ured using the BIOLOG-ECO technique.The results showed that the soil microbial activity assessed using well color development (AWCD)varied in the order:swamp meadow >alpine meadow >alpine shrub > cushion plants.Soil microbial communities in swamp meadow had the highest soil microbial activity;the carbon source utilization mode of soil microbial communities was similar under alpine meadow and swamp meadow.Correla-tion analysis indicated that the Shannon-Wiener index (H )was significantly correlated (P <0.05)with soil organic carbon and total nitrogen.Simpson’s (D )and Mclntosh’s (U )indices were significantly (P <0.05) correlated with soil total nitrogen.Redundancy analysis (RDA)on microbial communities constrained by dif-ferent soil factors showed that soil organic carbon,soil pH and total soil nitrogen were important factors deter-mining the rate of metabolism of carbon substrates in soils.The results of this study suggested a basis for fur-ther investigation of the relationships between vegetation diversity and soil microbial community diversity.