CAJ | 학술논문

研究柴胡皂苷（SSA）对体外发酵指标及发酵液中菌群变化的影响。采用完全随机试验设计，在0．5 g 全混合日粮（TMR）中分别添加0，0．125，0．25和0．5 mg 的 SSA，通过 AGRS-III 微生物发酵微量产气自动记录仪在39℃条件下进行48 h 体外发酵，并通过实时定量 PCR（RT－PCR）检测发酵液中菌群相对含量的变化。结果表明，0．25 g／kg 的 SSA 提高了干物质降解率（DMD，P ＝0．08）和总产气量（GP，P ＜0．05），并提高了发酵液中挥发酸（VFA）的含量（P ＜0．05），而0．5和1．0 g／kg 的 SSA 对 GP 和 DMD 没有显著的影响，但是提高了乙酸和 TVFA 的含量（P ＜0．05）。SSA 提高了白色瘤胃球菌和牛链球菌的相对含量（P ＜0．05）；但降低了短普雷沃氏菌和噬淀粉瘤胃杆菌的相对含量（P ＜0．05）。因此，SSA 提高了体外 GP 和 VFA 浓度，并改变发酵液中细菌的含量，这表明 SSA有利于调控微生物体外发酵参数。
연구시호조감（SSA）대체외발효지표급발효액중균군변화적영향。채용완전수궤시험설계，재0．5 g 전혼합일량（TMR）중분별첨가0，0．125，0．25화0．5 mg 적 SSA，통과 AGRS-III 미생물발효미양산기자동기록의재39℃조건하진행48 h 체외발효，병통과실시정량 PCR（RT－PCR）검측발효액중균군상대함량적변화。결과표명，0．25 g／kg 적 SSA 제고료간물질강해솔（DMD，P ＝0．08）화총산기량（GP，P ＜0．05），병제고료발효액중휘발산（VFA）적함량（P ＜0．05），이0．5화1．0 g／kg 적 SSA 대 GP 화 DMD 몰유현저적영향，단시제고료을산화 TVFA 적함량（P ＜0．05）。SSA 제고료백색류위구균화우련구균적상대함량（P ＜0．05）；단강저료단보뢰옥씨균화서정분류위간균적상대함량（P ＜0．05）。인차，SSA 제고료체외 GP 화 VFA 농도，병개변발효액중세균적함량，저표명 SSA유리우조공미생물체외발효삼수。
An experiment was conducted to investigate the effects of saikosaponin (SSA)on in vitro fermenta-tion parameters and bacterial quantity.Four treatments consisting of supplemental SSA at 0,0.25,0.5,and 1.0 g/kg dry matter (DM)were combined with 0.5 g total mixed ration (TMR),50 mL basal media and 25 mL rumen fluid obtained from ruminally cannulated,lactating Holstein dairy cows.The treatments were ran-domly assigned to 5 of 20 incubation bottles.Cumulative gas production (GP)was continuously monitored by an automated trace gas recording system (AGRS-III,Beijing)at 39℃ during the 48 h incubation.Relative con-tent of the bacterial community in the in vitro culture fluid was analyzed by real-time quantitative PCR.The results revealed that SSA at 0.25 g/kg could increase dry matter degradability (DMD,P =0.08),total gas production (GP,P <0.05)and the concentration of acetate,propionate and total volatile fatty acids (TVFA) (P <0.05).Adding SSA at 0.5 and 1.0 g/kg level did not affect GP kinetics and DMD,while it increased the concentration of acetate and TVFA (P <0.05).The supplement of SSA increased the relative quantity of Ru-minococcus albus and Streptococcus bovis (P <0.05),but decreased the content of Prevotella brevis and Rumi-nobacter amylophilus (P <0.05).Therefore,SSA supplementation improved gas production,increased VFA concentrations and the quantity of major microbial species in the in vitro culture fluid,which indicates that SSA may be beneficial for the manipulation of rumen microbial fermentation in vitro .