CAJ | 학술논문

目的：在pH＝7.40的tris－HCl缓冲溶液中研究头孢噻肟钠（ CTX）与牛血清白蛋白（ BSA）的相互作用。方法用荧光光谱测量BSA与CTX作用后的荧光强度，用Stern－Volmer方程、双对数方程和热力学方程对荧光强度进行计算。结果 CTX对BSA的荧光光谱产生猝灭作用，在299，307 K时，猝灭速率常数分别为3.07×1012，3.48×1012 L· mol－1· s－1，结合常数分别为5.75×103，4.47×103 L· mol－1，热力学参数分别为△G＜0、△H＜0和△S＜0。结论 CTX与BSA形成了基态复合物，两者之间的结合作用力主要为范德华力和氢键。
목적：재pH＝7.40적tris－HCl완충용액중연구두포새우납（ CTX）여우혈청백단백（ BSA）적상호작용。방법용형광광보측량BSA여CTX작용후적형광강도，용Stern－Volmer방정、쌍대수방정화열역학방정대형광강도진행계산。결과 CTX대BSA적형광광보산생졸멸작용，재299，307 K시，졸멸속솔상수분별위3.07×1012，3.48×1012 L· mol－1· s－1，결합상수분별위5.75×103，4.47×103 L· mol－1，열역학삼수분별위△G＜0、△H＜0화△S＜0。결론 CTX여BSA형성료기태복합물，량자지간적결합작용력주요위범덕화력화경건。
Objective To study the interaction between cefotaxime sodium for injection(CTX) and bovine serum albumin(BSA) in tris-HCl buffer of pH 7.40.Methods The fluorescence intensity of the interac-tion between BSA and CTX was studied by the fluorescence spectrometry.The fluorescence intensity were analyzed in according to Stern-Volmer equation, double logarithmic equation and thermodynamic equation.Results The fluorescence of BSA was quenched by CTX.At the 299 and 307 K, the quenching rate constant ( Kq ) was 3.07 ×1012 , 3.48 ×1012 L· mol-1 · s-1 ,and the binding constant(KA ) was 5.75 ×103 , 4.47 × 103 L · mol-1 , respectively.The value of thermodynamic parameters (△G,△H and△S) were less than zero.Conclusion The CTX and BSA formed the ground state complex, and the combining force between CTX and BSA was Van der Waals force and hydrogen bond.