CAJ | 학술논문

目的：观察6－苄氨基嘌呤（6－BA）对小鼠肝氧化损伤的保护作用。方法小鼠随机分为4组，对照组、模型组和低、高2个剂量实验组（20，40 mg· kg－16－BA）。单次腹腔注射CCl4制备小鼠肝损伤模型。造模前，实验组小鼠灌胃6－BA，连续7 d。检测小鼠肝总超氧化物歧化酶（ T－SOD）、谷胱甘肽过氧化物酶（ GSH－Px）活力和丙二醛（ MDA）含量；显微镜下观察形态结构和DNA氧化损伤水平。结果与模型组相比，低、高2个剂量实验组小鼠 T－SOD、GSH－Px活力显著提高，MDA含量显著降低。6－BA明显改善CCl4诱发的形态损伤和DNA损伤。结论6－BA对小鼠肝氧化损伤具有一定的保护作用。
목적：관찰6－변안기표령（6－BA）대소서간양화손상적보호작용。방법소서수궤분위4조，대조조、모형조화저、고2개제량실험조（20，40 mg· kg－16－BA）。단차복강주사CCl4제비소서간손상모형。조모전，실험조소서관위6－BA，련속7 d。검측소서간총초양화물기화매（ T－SOD）、곡광감태과양화물매（ GSH－Px）활력화병이철（ MDA）함량；현미경하관찰형태결구화DNA양화손상수평。결과여모형조상비，저、고2개제량실험조소서 T－SOD、GSH－Px활력현저제고，MDA함량현저강저。6－BA명현개선CCl4유발적형태손상화DNA손상。결론6－BA대소서간양화손상구유일정적보호작용。
Objective To study the protective effects of 6-benzylami-nopurine (6-BA) on oxidative injury in mice liver induced by carbon tetrachloride ( CCl4 ).Methods The mice were randomly divided into four groups, control group, model group, low dose test group, and high dose test group.Hepatic injury model was induced by once intraperito-neal injection of CCl4.Mice in test group were administrated by 6-BA (20, 40 mg · kg-1 ) by gavage once daily for 7 days before injecting CCl4.The activities of total-superoxide dismutase ( T-SOD) , glutathi-one peroxidase ( GSH -Px ) , and the content of malondialdehyde ( MDA ) were detected by spectrophotometer.Histological features of liver of mice were observed and DNA oxidative damage was measured by microscope.Results Compared with model group, pretreatment of 6-BA inhibited significantly MDA generation and increased the activities of T-SOD and GSH-Px, and improve significantly morphology and DNA damageinducedbyCCl4.Conclusion 6-BAhasprotectiveeffects against oxidative damage in liver tissue of mice induced by CCl4.