CAJ | 학술논문

目的：探讨细胞色素P450氧化酶（CYP）2C8和2J2基因多态性与中国汉族散发性阿尔茨海默病（ AD）的相关性。方法收集206例AD患者及256例健康对照的外周血标本，用全血基因组的提取试剂盒提取基因组的DNA，采用基质辅助激光解吸电离飞行时间质谱（ MALDI－TOF－MS）的方法，对CYP2C8（rs1058932，rs2275622）、CYP2J2（rs1155002，rs2271800）基因多态性进行检测。结果候选4个多态性的基因型和等位基因的频率分布在2组间比较差异均无统计学意义（ P＞0.05）。分别对CYP2C8／2J2的两个SNPs位点构建单体型， CYP2C8的单体型 C －T 与 AD 组呈正相关（ P ＜0.001， OR ＝2.57，95％CI：1.97～3.35）。结论 CYP2C8（rs1058932，rs2275622）单体型C－T增加了中国汉族散发性AD的发病风险。
목적：탐토세포색소P450양화매（CYP）2C8화2J2기인다태성여중국한족산발성아이자해묵병（ AD）적상관성。방법수집206례AD환자급256례건강대조적외주혈표본，용전혈기인조적제취시제합제취기인조적DNA，채용기질보조격광해흡전리비행시간질보（ MALDI－TOF－MS）적방법，대CYP2C8（rs1058932，rs2275622）、CYP2J2（rs1155002，rs2271800）기인다태성진행검측。결과후선4개다태성적기인형화등위기인적빈솔분포재2조간비교차이균무통계학의의（ P＞0.05）。분별대CYP2C8／2J2적량개SNPs위점구건단체형， CYP2C8적단체형 C －T 여 AD 조정정상관（ P ＜0.001， OR ＝2.57，95％CI：1.97～3.35）。결론 CYP2C8（rs1058932，rs2275622）단체형C－T증가료중국한족산발성AD적발병풍험。
Objective To detect the association between cytochrome P450 2C8 ( CYP2C8 ) and CYP2C9 polymorphisms and the risk of Alzheimer′s disease ( AD ) in Chinese Han population.Methods A case-control study was carried out to compare the distribution of geno-types and genetic frequencies of CYP2C8 and CYP2C9 polymorphisms between Alzheimer′s disease ( n =206 ) and healthy volunteers ( n=256 ) .Genomic DNA was extracted from whole blood using DNA blood Kit.Matrix-assisted laser desorption ionization time-of -flight mass spectrometry ( MALDI-TOF-MS) .Matrix-assisted laser desorp-tion ionization time-of-flight mass spectrometry ( MALDI-TOF-MS) was used to identify the polymorphisms of CYP2C8 and CYP2J2. Results There were no significant differences of CYP2C8 and CYP2C9 genotypes between the cases and control groups.The haplotype C-T of CYP2C8 associated with the AD group( P<0.001,OR=2.57,95%CI:1.97 -3.35 ) .Conclusion The haplotype C -T of CYP2C8 ( rs1058932, rs2275622 ) increased the risk of Alzheimer′s disease in Chinese Han population.