CAJ | 학술논문

目的：测定千金子水煎液中4种有效成分的含量，为千金子临床应用入煎剂提供依据。方法采用已建立的一测多评法测定千金子水煎液中3种二萜醇酯类成分千金子甾醇、千金子素L2、千金子素L3的含量，采用高效液相色谱法测定千金子水煎液中香豆素类成分秦皮乙素的含量。结果千金子打碎水煎液中千金子甾醇、千金子素L2、千金子素L3含量分别0.0159%、0.0059%、0.0241%，千金子整个入煎剂未检出以上3种成分；千金子打碎水煎液中秦皮乙素含量(0.6936%)＞千金子整个水煎液中秦皮乙素含量(0.2882%)。结论千金子中二萜醇酯类成分水煎溶出效果不佳，临床使用千金子泻下利尿时不宜水煎；千金子打碎水煎时秦皮乙素溶出效果较好，临床用于抗菌消炎可入煎剂。
목적：측정천금자수전액중4충유효성분적함량，위천금자림상응용입전제제공의거。방법채용이건립적일측다평법측정천금자수전액중3충이첩순지류성분천금자치순、천금자소L2、천금자소L3적함량，채용고효액상색보법측정천금자수전액중향두소류성분진피을소적함량。결과천금자타쇄수전액중천금자치순、천금자소L2、천금자소L3함량분별0.0159%、0.0059%、0.0241%，천금자정개입전제미검출이상3충성분；천금자타쇄수전액중진피을소함량(0.6936%)＞천금자정개수전액중진피을소함량(0.2882%)。결론천금자중이첩순지류성분수전용출효과불가，림상사용천금자사하이뇨시불의수전；천금자타쇄수전시진피을소용출효과교호，림상용우항균소염가입전제。
Objective To determine the contents of four effective constituents in Euphorbiae Semen decoction;To provide evidence for Euphorbiae Semen decoction into clinical application. Methods Established quantitative analysis multi-components by single marker method was used to determine the contents of diterpenoids constituents, such as euphorbia storoid, euphorbia factor L2, and euphorbia factor L3. HPLC method was used to determine the contents of aesculetin. Results Contents of euphorbia storoid, euphorbia factor L2, and euphorbia factor L3 in smashed Euphorbiae Semen decoction were 0.015 9%, 0.005 9% and 0.024 1%, respectively. However, the contents of the above three constituents could not be detected in whole Euphorbiae Semen decoction. The content of aesculetin (0.693 6%) in smashed Euphorbiae Semen decoction was more than that in whole Euphorbiae Semen decoction (0.288 2%). Conclusion Decoction digestion effect of diterpenoids constituents in Euphorbiae Semen decoction is not good. Decocting with water is not suitable for the clinical application of Euphorbiae Semen for purgation and diuresis. Aesculetin in smashed Euphorbiae Semen decoction has good decoction digestion effect, in which clinical use for antisepsis and anti-inflammation is effective.