CAJ | 학술논문

目的：对胶质芽孢杆菌PM13菌株的胞外多糖的单糖组成进行研究。方法取培养48 h的斜面菌体,用10倍体积的蒸馏水稀释,稀释液6000 r/min离心30 min后除去沉淀,上清液加4倍体积的95%的乙醇进行沉淀,并于6000 r/min离心30 min得沉淀物,沉淀物烘干得到多糖样品。采用苯酚-硫酸法测定多糖样品中含糖量。采用气相色谱法分析其单糖组成,色谱条件为：进样口温度为250℃,分流比为40：1, FID检测器温度为250℃, VF-5HT熔融石英毛细柱,柱温为160℃,8℃/min升至200℃,200℃保持5 min,载气为高纯氮气,1.6 mL/min。结果 PM13菌株胞外多糖的含糖量为90.98%,其单糖组成为葡萄糖、半乳糖、甘露糖和木糖,比例为5.57：3.08：4.04：1。结论本文建立了用气相色谱法检测胶质芽孢杆菌PM13菌株胞外多糖的单糖组成的方法,确定了胶质芽孢杆菌胞外多糖的单糖组成及相应比例。
목적：대효질아포간균PM13균주적포외다당적단당조성진행연구。방법취배양48 h적사면균체,용10배체적적증류수희석,희석액6000 r/min리심30 min후제거침정,상청액가4배체적적95%적을순진행침정,병우6000 r/min리심30 min득침정물,침정물홍간득도다당양품。채용분분-류산법측정다당양품중함당량。채용기상색보법분석기단당조성,색보조건위：진양구온도위250℃,분류비위40：1, FID검측기온도위250℃, VF-5HT용융석영모세주,주온위160℃,8℃/min승지200℃,200℃보지5 min,재기위고순담기,1.6 mL/min。결과 PM13균주포외다당적함당량위90.98%,기단당조성위포도당、반유당、감로당화목당,비례위5.57：3.08：4.04：1。결론본문건립료용기상색보법검측효질아포간균PM13균주포외다당적단당조성적방법,학정료효질아포간균포외다당적단당조성급상응비례。
Objective To study the monosaccharide composition of the exopolysaccharides produced by Bacillus mucilaginosus PM13. Method Bacillus mucilaginosus PM13 was cultured on agar slant for 48 h, diluted by 10 times volume of distilled water, centrifuged at 6000 r/min for 30 min. The supernatant was collected and mixed with 4 times volume of 95%ethanol, and centrifuged at 6000 r/min for 30 min to obtain precipitate. The precipitate was dried to obtain the polysaccharide sample. The content of sugar was determined by phenol sulfuric acid method. The monosaccharide composition was analyzed by gas chromatography. The chromatographic condition was as follows: injector temperature 250 ℃, spilt ratio 40:1, FID detector temperature 250℃, VF-5HT melted silica capillary column. The initial column temperature was 160℃, then increased to 200 ℃ with the rate of 8 ℃/min and kept this temperature for 5 min. The carrier gas was high purity nitrogen gas with the speed of 1.6 mL/min. Results The sugar content of sample was 90.98% on average. The results of the gas chromatography analysis showed that the polysaccharide produced by PM13 strain was mainly composed of glucose, galactose, mannose, and xylose with the ratio of 5.57:3.08:4.04:1. Conclusion The detection method of the monosaccharide composition of the exopolysaccharides from Bacillus mucilaginosus PM13 by gas chromatography is established and the monosaccharide composition and the rate are obtained.