CAJ | 학술논문

目的：观察化浊颗粒对2型糖尿病(T2DM)大鼠磷脂酰肌醇3激酶(PI-3K)、葡萄糖转运蛋白4(GLUT4)在骨骼肌组织中表达的影响,探讨其对PI-3K及GLUT4信号通路激活的作用。方法采用高脂饲料喂养联合小剂量链脲佐菌素腹腔注射建立T2DM大鼠模型,随机分为化浊颗粒组、罗格列酮组、模型组。各给药组给予相应药物灌胃,模型组与空白组予生理盐水灌胃,观察大鼠一般状态、体质量及摄食量。干预10周后,予10%水合氯醛麻醉大鼠,检测大鼠空腹血糖(FBG)、葡萄糖耐量试验餐后2 h血糖(2 h PG)、空腹胰岛素(FINS)水平；RT-PCR检测各组大鼠骨骼肌组织PI-3K和GLUT4 mRNA表达的水平。结果与模型组比较,化浊颗粒组与罗格列酮组大鼠FBG、2 h PG及FINS水平降低(P＜0.05),PI-3K及GLUT4 mRNA表达水平升高(P＜0.05)。结论化浊颗粒具有提高胰岛素敏感性的作用,与激活骨骼肌组织胰岛素信号传导通路中PI-3K和GLUT4 mRNA的表达有关。
목적：관찰화탁과립대2형당뇨병(T2DM)대서린지선기순3격매(PI-3K)、포도당전운단백4(GLUT4)재골격기조직중표체적영향,탐토기대PI-3K급GLUT4신호통로격활적작용。방법채용고지사료위양연합소제량련뇨좌균소복강주사건립T2DM대서모형,수궤분위화탁과립조、라격렬동조、모형조。각급약조급여상응약물관위,모형조여공백조여생리염수관위,관찰대서일반상태、체질량급섭식량。간예10주후,여10%수합록철마취대서,검측대서공복혈당(FBG)、포도당내량시험찬후2 h혈당(2 h PG)、공복이도소(FINS)수평；RT-PCR검측각조대서골격기조직PI-3K화GLUT4 mRNA표체적수평。결과여모형조비교,화탁과립조여라격렬동조대서FBG、2 h PG급FINS수평강저(P＜0.05),PI-3K급GLUT4 mRNA표체수평승고(P＜0.05)。결론화탁과립구유제고이도소민감성적작용,여격활골격기조직이도소신호전도통로중PI-3K화GLUT4 mRNA적표체유관。
Objective To observe the effects of Huazhuo Granule on expression of phosphatidylinositol-3-kinase (PI-3K) and glucose transporter 4 (GLUT4) in skeletal muscle type 2 diabetes (T2DM) rats;To discuss its effects on activating signal path of PI-3K and GLUT4. Methods High-fat diet combined with low-dose streptozotocin intraperitoneal injection was used to establish T2DM rat models. The rats were randomly divided into blank group, Huazhuo Granule group, rosiglitazone group, and model group. Each treatment group was given relevant medicine for gavage. Model group and blank control group were given normal saline for gavage. The general state, weight, and food ration of rats were observed. After ten-week medicine intervention, 10%chloral hydrate was used for anesthesia to detect the levels of FBG, OGTT (2 h PG), and FINS. RT-PCR was used to detect the expressions of PI-3K and GLUT4 mRNA of rat skeletal muscle in all groups. Results Compared with model group, the levels of FBG, 2 h PG, and FINS decreased (P<0.05);the expressions of PI-3K and GLUT4 mRNA of rat skeletal muscle increased significantly (P<0.05). Conclusion Huazhuo Granule has the effects on increasing insulin sensitivity, which is related to activating insulin signal path of PI-3K and GLUT4 of skeletal muscle tissues.