食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
6期
1973-1979
,共7页
曹佩琴%牛丽%黄建安%刘仲华
曹珮琴%牛麗%黃建安%劉仲華
조패금%우려%황건안%류중화
朝鲜蓟叶水提取物%HepG2细胞%酒精性肝细胞损伤
朝鮮薊葉水提取物%HepG2細胞%酒精性肝細胞損傷
조선계협수제취물%HepG2세포%주정성간세포손상
artichoke leaf extractive%HepG2 cells%alcohol-induced liver cell damage
目的：通过建立酒精损伤HepG2细胞模型,探讨朝鲜蓟叶水提取物对酒精诱导的HepG2细胞损伤的影响。方法以人肝癌细胞株 HpeG2为实验材料,采用酒精加入细胞培养液培养 HepG2细胞,建立酒精损伤HepG2细胞模型,以MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide)法筛选酒精处理浓度、时间以及朝鲜蓟叶水提取物最佳作用浓度,通过检测各组细胞培养基中谷丙转氨酶(alanine aminotran-sferase, ALT)、谷草转氨酶(aspartate transaminase, AST)和细胞内超氧化物歧化酶(superoxide dismutase, SOD)、丙二醛(malondialdehyde, MDA)、甘油三酯(triglyceride, TG)的活性,来评价朝鲜蓟叶水提取物对酒精诱导的HepG2肝细胞损伤的影响。结果分别以0.6%、1.2%、2.4%的酒精浓度联合2.5、5、10μg/mL朝鲜蓟叶水提取物培养HepG2细胞48 h。模型组随着酒精浓度增高,细胞的AST、ALT、TG、MDA水平明显升高, SOD活性显著下降。处理组随着朝鲜蓟叶水提取物使用浓度的增加,细胞中AST、ALT、TG、MDA水平与模型组相比明显下降, SOD活性升高,对HepG2细胞的保护作用增强。结论朝鲜蓟叶水提取物在一定程度上能保护酒精受损的HepG2细胞,具有深入研究和开发的潜能。
目的：通過建立酒精損傷HepG2細胞模型,探討朝鮮薊葉水提取物對酒精誘導的HepG2細胞損傷的影響。方法以人肝癌細胞株 HpeG2為實驗材料,採用酒精加入細胞培養液培養 HepG2細胞,建立酒精損傷HepG2細胞模型,以MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide)法篩選酒精處理濃度、時間以及朝鮮薊葉水提取物最佳作用濃度,通過檢測各組細胞培養基中穀丙轉氨酶(alanine aminotran-sferase, ALT)、穀草轉氨酶(aspartate transaminase, AST)和細胞內超氧化物歧化酶(superoxide dismutase, SOD)、丙二醛(malondialdehyde, MDA)、甘油三酯(triglyceride, TG)的活性,來評價朝鮮薊葉水提取物對酒精誘導的HepG2肝細胞損傷的影響。結果分彆以0.6%、1.2%、2.4%的酒精濃度聯閤2.5、5、10μg/mL朝鮮薊葉水提取物培養HepG2細胞48 h。模型組隨著酒精濃度增高,細胞的AST、ALT、TG、MDA水平明顯升高, SOD活性顯著下降。處理組隨著朝鮮薊葉水提取物使用濃度的增加,細胞中AST、ALT、TG、MDA水平與模型組相比明顯下降, SOD活性升高,對HepG2細胞的保護作用增彊。結論朝鮮薊葉水提取物在一定程度上能保護酒精受損的HepG2細胞,具有深入研究和開髮的潛能。
목적：통과건립주정손상HepG2세포모형,탐토조선계협수제취물대주정유도적HepG2세포손상적영향。방법이인간암세포주 HpeG2위실험재료,채용주정가입세포배양액배양 HepG2세포,건립주정손상HepG2세포모형,이MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide)법사선주정처리농도、시간이급조선계협수제취물최가작용농도,통과검측각조세포배양기중곡병전안매(alanine aminotran-sferase, ALT)、곡초전안매(aspartate transaminase, AST)화세포내초양화물기화매(superoxide dismutase, SOD)、병이철(malondialdehyde, MDA)、감유삼지(triglyceride, TG)적활성,래평개조선계협수제취물대주정유도적HepG2간세포손상적영향。결과분별이0.6%、1.2%、2.4%적주정농도연합2.5、5、10μg/mL조선계협수제취물배양HepG2세포48 h。모형조수착주정농도증고,세포적AST、ALT、TG、MDA수평명현승고, SOD활성현저하강。처리조수착조선계협수제취물사용농도적증가,세포중AST、ALT、TG、MDA수평여모형조상비명현하강, SOD활성승고,대HepG2세포적보호작용증강。결론조선계협수제취물재일정정도상능보호주정수손적HepG2세포,구유심입연구화개발적잠능。
Objective To investigate the effect of artichoke (Cynara scolymus L.) leaf extract on alcohol-induced HepG2 cell damage by the establishment of alcohol damage HepG2 liver cell model. Methods Alohol-induced HepG2 cell damage model was established by adding alcohol to the cell culture medium of HepG2 cell and using hepatoma cell line. MTT(3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay was used to determine the optimal alcohol concentration, treating time and the artichoke leaf extract concentration. The effect of artichoke leaf extract on alcohol-induced HepG2 cell damage was evaluated by detecting the activity of intracellular ALT (alanine aminotransferase), AST (aspartate transaminase) and SOD (superoxide dismutase), the content of MDA (malondialdehyde) and TG (triglyceride) in each group of cell culture medium. Resules HepG2 cells were treated by 0.6%, 1.2%, and 2.4%alcohol concentration with 2.5, 5, and 10μg/mL artichoke leaf extract for 48 h. Model group treated with the alcohol concentration, cell AST, ALT, TG, MDA levels were significantly increased and SOD activity decreased. With the increase of cell concentrations, the AST, ALT, TG and MDA level significantly decreased in treatment group comparing with the model group, SOD activity increased, and the protective effect on HepG2 cells increased. Conclusion Artichoke leaf extract can protect the alcohol impaired HepG2 cells to some extent, which has the potential of intensive research and development.
