CAJ | 학술논문

目的：探讨紫柏凝胶药物血清作用于高危型人乳头瘤病毒感染相关性宫颈癌SiHa细胞的免疫学反应机制。方法采用细胞培养及免疫组化方法,用不同浓度紫柏凝胶药物血清作用宫颈癌SiHa细胞24、48、72 h 后,分别标记出相同作用时间段不同浓度下及不同时间段相同浓度下白细胞介素(IL)-6、IL-10、CD83、肿瘤坏死因子-α(TNF-α)表达的光密度,通过计算平均光密度,分析药物作用与免疫因子 IL-6、IL-10、CD83、TNF-α表达的量效与时效关系。结果随着药物血清浓度及作用时间的增加,IL-6及TNF-α的免疫表达均逐渐减弱,CD83及IL-10的免疫表达均逐渐增强(P＜0.05)。结论紫柏凝胶药物血清能够通过改善宫颈局部免疫功能,抑制局部炎症反应,从而抑制宫颈高危型人乳头瘤病毒。
목적：탐토자백응효약물혈청작용우고위형인유두류병독감염상관성궁경암SiHa세포적면역학반응궤제。방법채용세포배양급면역조화방법,용불동농도자백응효약물혈청작용궁경암SiHa세포24、48、72 h 후,분별표기출상동작용시간단불동농도하급불동시간단상동농도하백세포개소(IL)-6、IL-10、CD83、종류배사인자-α(TNF-α)표체적광밀도,통과계산평균광밀도,분석약물작용여면역인자 IL-6、IL-10、CD83、TNF-α표체적량효여시효관계。결과수착약물혈청농도급작용시간적증가,IL-6급TNF-α적면역표체균축점감약,CD83급IL-10적면역표체균축점증강(P＜0.05)。결론자백응효약물혈청능구통과개선궁경국부면역공능,억제국부염증반응,종이억제궁경고위형인유두류병독。
Objective To investigate the immunological reaction mechanism of medicated serum in Zibai Gelatin for SiHa cells of the cervical cancer infected by high risk human papilloma virus (HPV). Methods Through immunohistochemical comparison and the cell culture, and after medicated serum was administrated to SiHa cells of cervical cancer for 24 h, 48 h, and 72 h, optical densities of IL-6, IL-10, CD83 and TNF-αin the same time but different concentrations and different times but the same concentration were observed. Relationships of dose-effect and time-effect between expressions of IL-6, IL-10, CD83 and TNF-α and medicine action were analyzed by calculating average optical density. Results With the increase of medicine concentration and administration time of Zibai Gelatin, the expressions of IL-6 and TNF-αgradually decreased, while the expressions of CD83 and IL-10 gradually increased (P<0.05). Conclusion Zibai Gelatin with medicated serum can inhibit local inflammatory reaction by improving local immunologic function of cervix, which is beneficial to reduce cervix high-risk HPV.