中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2015年
7期
47-50
,共4页
田静%李忻红%朱卉雯%马贤德%冯常青%王敏
田靜%李忻紅%硃卉雯%馬賢德%馮常青%王敏
전정%리흔홍%주훼문%마현덕%풍상청%왕민
健脾养血祛风方%脾虚%慢性湿疹%水通道蛋白3%小鼠
健脾養血祛風方%脾虛%慢性濕疹%水通道蛋白3%小鼠
건비양혈거풍방%비허%만성습진%수통도단백3%소서
Jianpi Yangxue Qufeng Formula%spleen deficiency%chronic eczema%AQP3%mice
目的:观察健脾养血祛风方对脾虚慢性湿疹模型小鼠皮损处水通道蛋白3(AQP3)含量的影响,并探讨其可能的作用机制。方法取健康雄性小鼠50只,随机分为正常组、模型组、阳性药组及中药高、低剂量组。采用小剂量反复 DNCB 涂抹及番泻叶致脾虚证建立小鼠脾虚慢性湿疹模型。中药组予健脾养血祛风方灌胃,阳性药组予盐酸左西替利嗪灌胃。免疫组化法检测病变皮肤组织中 AQP3的表达,同时观察皮肤组织病理变化。结果病理观察结果显示,健脾养血祛风方对小鼠皮损处炎症损伤具有一定的修复作用。与正常组比较,模型组AQP3过度表达;与模型组比较,各给药组AQP3表达均明显下降,染色强度降低。模型组AQP3平均光密度值明显高于正常组(P<0.01);与模型组比较,各给药组均能下调AQP3表达(P<0.05)。结论健脾养血祛风方下调皮损中过度表达的AQP3可能是其治疗脾虚慢性湿疹的作用机制之一。
目的:觀察健脾養血祛風方對脾虛慢性濕疹模型小鼠皮損處水通道蛋白3(AQP3)含量的影響,併探討其可能的作用機製。方法取健康雄性小鼠50隻,隨機分為正常組、模型組、暘性藥組及中藥高、低劑量組。採用小劑量反複 DNCB 塗抹及番瀉葉緻脾虛證建立小鼠脾虛慢性濕疹模型。中藥組予健脾養血祛風方灌胃,暘性藥組予鹽痠左西替利嗪灌胃。免疫組化法檢測病變皮膚組織中 AQP3的錶達,同時觀察皮膚組織病理變化。結果病理觀察結果顯示,健脾養血祛風方對小鼠皮損處炎癥損傷具有一定的脩複作用。與正常組比較,模型組AQP3過度錶達;與模型組比較,各給藥組AQP3錶達均明顯下降,染色彊度降低。模型組AQP3平均光密度值明顯高于正常組(P<0.01);與模型組比較,各給藥組均能下調AQP3錶達(P<0.05)。結論健脾養血祛風方下調皮損中過度錶達的AQP3可能是其治療脾虛慢性濕疹的作用機製之一。
목적:관찰건비양혈거풍방대비허만성습진모형소서피손처수통도단백3(AQP3)함량적영향,병탐토기가능적작용궤제。방법취건강웅성소서50지,수궤분위정상조、모형조、양성약조급중약고、저제량조。채용소제량반복 DNCB 도말급번사협치비허증건립소서비허만성습진모형。중약조여건비양혈거풍방관위,양성약조여염산좌서체리진관위。면역조화법검측병변피부조직중 AQP3적표체,동시관찰피부조직병리변화。결과병리관찰결과현시,건비양혈거풍방대소서피손처염증손상구유일정적수복작용。여정상조비교,모형조AQP3과도표체;여모형조비교,각급약조AQP3표체균명현하강,염색강도강저。모형조AQP3평균광밀도치명현고우정상조(P<0.01);여모형조비교,각급약조균능하조AQP3표체(P<0.05)。결론건비양혈거풍방하조피손중과도표체적AQP3가능시기치료비허만성습진적작용궤제지일。
Objective To observe the effects of Jianpi Yangxue Qufeng Formula (JPYXQF) on the AQP3 in mice with chronic eczema, and explore mechanism of action. Methods Fifty healthy male mice were randomly divided into 5 groups, namely normal group, model group, positive medicine group and JPYXQF high and low dose groups. Low-dose DNCB and Sennae Fominm were used to establish mice models of chronic eczema with spleen deficiency. JPYXQF groups were treated by JPYXQF for gavage, while the positive medicine group was treated by levocetirizine hydrochloride for gavage. The expression of AQP3 in mice skin tissue was detected by immunohistochemical method. At the same time, the pathological changes of skin were observed. Results The pathology of mice skin lesion showed that JPYXQF has certain recovery effects on the inflammation injury of skin lesion. Compared with the normal group, expression of AQP3 over expressed in model group. Compared with the model group, the expression of AQP3 in all treatment groups significantly decreased, and the staining intensity decreased. In the model group, the average optical density of AQP3 was significantly higher than that in the normal group (P<0.01). Compared with the model group, the treatment groups can reduce the expression of AQP3 in mice skin tissues (P<0.05). Conclusion JPYXQF can reduce the over expression of AQP3 in skin lesion, which is probably its mechanism for the treatment of chronic eczema.