CAJ | 학술논문

利用电聚合方法制备聚邻苯二胺修饰碳糊电极（CPE／POPD ），在CPE／POPD上电沉积金‐普鲁士蓝纳米复合材料（Au‐PB），制得CPE／POPD／Au‐PB修饰电极，将羊抗小鼠IgG（抗体，Ab）通过Au‐NH‐键固定在修饰电极上，从而制得免疫传感器（CPE／POPD／Au‐PB／Ab）．利用CPE／POPD／Au‐PB／Ab对小鼠IgG （抗原，Ag ）进行检测，结果表明，在4×10－4～1．0×104μg／L质量浓度范围内，电极电势与Ag浓度呈现良好线性关系，检测下限达到1．14×10－4μg／L ，响应时间为3 min ，灵敏度为－8．51 mV／dec‐ade ．所制备的免疫传感器具有灵敏度高、响应速度快、检测范围宽、重现性好等优良性能．
이용전취합방법제비취린분이알수식탄호전겁（CPE／POPD ），재CPE／POPD상전침적금‐보로사람납미복합재료（Au‐PB），제득CPE／POPD／Au‐PB수식전겁，장양항소서IgG（항체，Ab）통과Au‐NH‐건고정재수식전겁상，종이제득면역전감기（CPE／POPD／Au‐PB／Ab）．이용CPE／POPD／Au‐PB／Ab대소서IgG （항원，Ag ）진행검측，결과표명，재4×10－4～1．0×104μg／L질량농도범위내，전겁전세여Ag농도정현량호선성관계，검측하한체도1．14×10－4μg／L ，향응시간위3 min ，령민도위－8．51 mV／dec‐ade ．소제비적면역전감기구유령민도고、향응속도쾌、검측범위관、중현성호등우량성능．
A novel potentiometric immunosensor has been presented in this paper .O‐phenylenediamine (OPD) was firstly polymerized on a carbon paste electrode (CPE) by cyclic voltammetry method ,then gold‐Prussian blue nanocomposite (Au‐PB) was electrochemically deposited onto the CPE/POPD to fabricate CPE/POPD/GNPs‐PB .Finally ,the goat anti‐mouse IgG (antibody ,Ab) were self‐assem‐bled noto the CPE/POPD/GNPs‐PB through the Au‐NH‐bond to form a potentiometric immunosen‐sor (CPE /POPD/GNPs‐PB/Ab) .Under optimal conditions ,the resulting immunosensor displayed a rapid response to antigen (mouse IgG) and the linear range of the immunosensor was from 4 .0 × 10-4 to 1 .0 × 104 μg/L (correlation coefficient r=0 .998) with a lower detection limit of 1 .14 × 10-4 μg/L and a sensitivity of 8 .51 mV/decade .Furthermore ,the immunosensor exhibited good reproducibili‐ty ,acceptable stability ,and low cost .