CAJ | 학술논문

利用乙酰半胱氨酸分子中的巯基(-SH)可将Fe( III)还原为Fe( II),Fe( II)与K3[ Fe( CN)6]反应生成在720 nm处有最大吸收的可溶性普鲁士蓝KFe[ Fe( CN)6],通过测定普鲁士蓝的吸光度,可间接测定乙酰半胱氨酸的含量,从而建立了准确、快速测定乙酰半胱氨酸含量的新方法。乙酰半胱氨酸质量浓度在0．6560~6．560μg·mL-1范围内与A呈良好的线性关系,线性回归方程为A=0．00940+0．0803 C(μg·mL-1),线性相关系数r=0．9996,表观摩尔吸收系数ε=1．1×104 L/(mol·cm)。方法用于实际药品中乙酰半胱氨酸的含量测定,结果与药典法(高效液相色谱法)一致。
이용을선반광안산분자중적구기(-SH)가장Fe( III)환원위Fe( II),Fe( II)여K3[ Fe( CN)6]반응생성재720 nm처유최대흡수적가용성보로사람KFe[ Fe( CN)6],통과측정보로사람적흡광도,가간접측정을선반광안산적함량,종이건립료준학、쾌속측정을선반광안산함량적신방법。을선반광안산질량농도재0．6560~6．560μg·mL-1범위내여A정량호적선성관계,선성회귀방정위A=0．00940+0．0803 C(μg·mL-1),선성상관계수r=0．9996,표관마이흡수계수ε=1．1×104 L/(mol·cm)。방법용우실제약품중을선반광안산적함량측정,결과여약전법(고효액상색보법)일치。
Fe(Ⅲ) can be reduced to Fe(Ⅱ) by hydrosulfuryl group(-SH) in acetylcysteine molecule,and then Fe(Ⅱ) reacted with potassium ferricyanide K3 [ Fe( CN) 6 ] to form soluble prussian blue( KFe[ Fe( CN) 6 ] ) which its maximum absorption wavelength is 720 nm,the content of acetylcysteine was indirectly by determinated the absorbance of the soluble prussian blue. An accurate and fast spectrophotometric method for the determination the content of acetylcysteine had been established. A good linear relationship is obtained between the absorbance and the concentration of acetylcysteine in the range of 0. 6560~6. 560μg·mL-1 ,and the equation of the linear regression is A=0. 00940+0. 0803C(μg·mL-1),with a linear correlation coefficient is 0. 9996. The molar absorbancy index is 1. 1×104 L/(mol·cm). This proposed method has been applied to determine the content of acetylcysteine in real pharma-ceuticals,and the results agreed well with pharmacopoeial method( HPLC) .